Evidence mapPaperPMID 42449970Full record

ReviewInternational journal of molecular sciences2026

Thermostability Engineering in Therapeutic Antioxidant Enzymes: From Molecular Fundamentals to Oxidative Stress Applications.

Diana Tatarciuc, Irina Mihaela Esanu, Iolanda Foia, Mioara-Florentina Trandafirescu, Teodor Flaviu Vasilcu, Dragos Catalin Ghica, Magda Ecaterina Antohe, Adina Oana Armencia, Roxana Ionela Vasluianu

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Diana TatarciucGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Irina Mihaela EsanuGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Iolanda FoiaGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Mioara-Florentina TrandafirescuGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Teodor Flaviu VasilcuGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Dragos Catalin GhicaGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.ORCID 0000-0002-9721-6582
Magda Ecaterina AntoheGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Adina Oana ArmenciaGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.ORCID 0000-0003-0921-4382
Roxana Ionela VasluianuGrigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.ORCID 0000-0003-0867-1135

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The efficacy of enzyme therapy is limited by their poor stability under physiological conditions. Thermostable enzymes, derived from extremophilic organisms or generated by advanced protein engineering, offer a revolutionary solution to this long-standing challenge. They are widely used in industrial biocatalysis. Their therapeutic applications are poorly investigated and spread across diverse disciplines. While most applications are in the preclinical stages, emerging evidence from animal models demonstrates proof-of-concept for thermostable antioxidant enzymes in cardiovascular, neurodegenerative, and inflammatory diseases. This review critically assesses the translational landscape, distinguishing between established therapeutic enzymes (e.g., asparaginase, PEGylated SOD) and emerging experimental candidates. This narrative review consolidates existing knowledge about thermostable enzyme engineering and their emerging functions as molecular therapies, particularly in oxidative stress-related diseases. This review synthesizes recent advances in structural biology, computational protein design, biomaterials engineering, and translational antioxidant strategies, highlighting how breaking down disciplinary barriers is accelerating the development of sustainable and self-regenerating antioxidant platforms. By integrating molecular precision with systems-level therapeutic design, engineered thermostable antioxidant enzymes exemplify the future of biological development, where multidisciplinary collaboration drives innovation against oxidative stress-driven pathologies. Engineered thermostable enzymes provide a versatile basis for next-generation therapeutics, with the potential to address medical needs through improved stability, targeted activity, and multifunctional design.

Indexed as

AntioxidantsEnzymesOxidative StressProtein EngineeringAnimalsEnzyme StabilityHumansAntioxidantsEnzymesantioxidant enzymescomputational designoxidative stressprotein engineeringthermostable enzymes

Identifiers

PMID42449970
PMCPMC13361086

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.