Investigating the Potential Mechanism of Oxymatrine in Alleviating Heat Stress Injury Based on Network Pharmacology, Molecular Docking, and In Vitro Validation.

Sheng Cheng, Xingxing Song, Wenying Qiu, Xiaowan Liu, Guangneng Peng, Jialiang Xin

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

6 authors.

Sheng ChengKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0009-0003-1204-2873

Xingxing SongKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Wenying QiuKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Xiaowan LiuKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Guangneng PengKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0000-0002-9898-5754

Jialiang XinKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Funding

Guangneng Peng 2024YFD1800200

6 · The paper itself

Abstract

Global warming has increasingly positioned heat stress (HS) as a major threat to public health, as it can inflict damage on multiple organs including the kidneys, liver, and heart. However, effective targeted therapeutic strategies remain limited. This investigation employed an integrated approach combining Network pharmacology, in silico binding simulations, and cell-based assays to elucidate the cytoprotective properties and molecular basis of oxymatrine action under heat-stressed conditions. Network analysis identified 36 overlapping targets common to oxymatrine and the pathological processes of HS-related acute kidney injury (AKI), acute liver injury (ALI), and acute myocardial injury (AMI). These targets were strongly enriched in the PI3K-AKT signaling cascade. Molecular docking showed that oxymatrine binds tightly to key pathway proteins such as PIK3CA and GSK3B, with Vina scores below -8 kcal/mol. In 293T cells, the half-maximal cytotoxic concentration (CC

Indexed as

AlkaloidsHeat-Shock ResponseQuinolizinesAnimalsCell SurvivalClass I Phosphatidylinositol 3-KinasesGlycogen Synthase Kinase 3 betaHEK293 CellsHumansMatrinesMolecular Docking SimulationNetwork PharmacologyPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionAlkaloidsClass I Phosphatidylinositol 3-KinasesGlycogen Synthase Kinase 3 betaMatrinesoxymatrinePhosphatidylinositol 3-KinasesPIK3CA protein, humanProto-Oncogene Proteins c-aktQuinolizinesferroptosisheat stressmolecular dockingnetwork pharmacologyoxymatrinePI3K-AKT signaling pathway

Identifiers

PMID42450186

DOI10.3390/ijms27135919

PMCPMC13362203

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.