ArticleInternational journal of molecular sciences2026
Longstanding Transcriptional Activation of APOA1 and PON1 in Human Hepatocytes by CRISPR/dCas9 Technology: Transcriptomic Profile and Crosstalk with Endothelial Cells.
Jessica I C Haratau, Loredan S Niculescu, Teodora Barbalata, Gabriela M Sanda, Elena V Fuior, Shlomo Sasson, Anca V Sima, Camelia S Stancu, Laura Toma
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In one paragraphArticle in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
9 authors.
Jessica I C HaratauLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.
Loredan S NiculescuLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.ORCID 0000-0002-1394-9085 Teodora BarbalataLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.ORCID 0000-0003-1621-4233 Gabriela M SandaLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.
Elena V FuiorGene Regulation and Molecular Therapies Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.ORCID 0000-0001-9166-8417 Shlomo SassonDepartment of Pharmacology, Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112002, Israel.ORCID 0000-0002-9343-526X Anca V SimaLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.
Camelia S StancuLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.
Laura TomaLipidomics Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Haşdeu Street, 050568 Bucharest, Romania.
Funding
EU - PNRR Programme PNRR-III-C9-2022-I8-197Ministry of Education and Research, CCCDI - UEFISCDI PN-IV-P6-6.1-CoEx-2024-0029
6 · The paper itselfAbstract
Apolipoprotein A1 (APOA1) and paraoxonase 1 (PON1) are key proteins of high-density lipoproteins (HDL). The aim of the present study was to obtain and characterize an in vitro model for endogenous APOA1 and PON1 longstanding upregulation in hepatocytes that can be further used to decipher the mechanisms of their protective action. Cultured human hepatocytes (HuH-7 cell line) were transfected with CRISPR/dCas9 activation plasmids targeting
Indexed as
Apolipoprotein A-IAryldialkylphosphataseCRISPR-Cas SystemsHepatocytesTranscriptional ActivationTranscriptomeEndothelial CellsGene Expression ProfilingHumansHuman Umbilical Vein Endothelial CellsOxidative StressTumor Necrosis Factor-alphaAPOA1 protein, humanApolipoprotein A-IAryldialkylphosphatasePON1 protein, humanTumor Necrosis Factor-alphaapolipoprotein A1CRISPR/dCas9hepatocytesin vitro experimental modelparaoxonase 1primary endothelial cellsRNAseqtranscriptomic profile
Identifiers
PMID42450220
PMCPMC13361997
What Socratic holds
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