Unraveling the Dynamic Evolution of Volatile Aroma Compounds in Sea Buckthorn-Grape Composite Fruit Wine During Sequential Yeast-Lactic Acid Bacteria Fermentation.

Sea buckthorn is rich in functional components but features high acidity and susceptibility to oxidative deterioration, leading to flavor defects such as sour-astringent taste and rancidity in single-fruit wine. Co-fermentation is an effective strategy for flavor and nutrition complementarity, but the dynamic evolution of volatile aroma during yeast-lactic acid bacteria combined fermentation of such wine remains unclear. This study employed 'Zhuangyuan Huang' sea buckthorn and 'Marselan' grape to produce the composite fruit wine via sequential co-fermentation, and systematically investigated dynamic aroma changes using HS-SPME-GC-MS, orthogonal partial least squares discriminant analysis (OPLS-DA), and relative odor activity value (ROAV) analysis. Results revealed that post-fermentation, total detected volatile compounds increased from 90 to 118, with esters and alcohols rising by 24 and 11 respectively, serving as core contributors to enhanced aroma richness. ROAV analysis demonstrated this process significantly reduced the contribution of off-flavor acids, boosted the importance of floral, fruity, and sweet compounds, and elevated the sensory score from 26.8 to 84.1. OPLS-DA further confirmed significant inter-stage aroma differences with excellent intergroup discrimination. These findings confirm that this sequential fermentation breaks processing bottlenecks of high-acid fruits, reveals the synergistic flavor-modulating effects of multi-microbial sequential fermentation, and provides theoretical support for process optimization and high-value processing of composite fruit wine.