Evidence mapPaperPMID 42450802Full record

ArticleAnimals : an open access journal from MDPI2026

Selenium Attenuates LPS-Induced Injury in Ovine Granulosa Cells by Protecting Mitochondrial Ultrastructure and Cellular Homeostasis.

Zeyuan Guo, Jun Li, Xinyu Fan, Yufei Liu, Linzhen Li, Lihua Lyu, Chunhe Yang, Youshen Ren

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zeyuan GuoCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Jun LiCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Xinyu FanCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Yufei LiuCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Linzhen LiCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Lihua LyuCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.ORCID 0000-0002-4481-2882
Chunhe YangCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Youshen RenCollege of Animal Science, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.ORCID 0000-0002-0984-6198

Funding

National Key R&D Program of China 2024YFD1301004
6 · The paper itself

Abstract

Lipopolysaccharide (LPS) impairs the function of ovine follicular granulosa cells (GCs), representing a primary cause of follicular atresia. Selenium (Se), an essential trace element, possesses anti-inflammatory and cytoprotective properties; however, its effects on GC ultrastructure remain largely unknown. In this study, primary ovine GCs were exposed to LPS (10 µg/mL) and treated with sodium selenite (25 nM). Transmission electron microscopy (TEM), JC-1 staining, enzyme-linked immunosorbent assay (ELISA), reactive oxygen species (ROS) detection, flow cytometry, and quantitative real-time PCR (qRT-PCR) were employed to evaluate cellular ultrastructure, mitochondrial membrane potential (ΔΨm), and downstream physiological processes. LPS induced severe mitochondrial pyknosis, cristae loss, and reduced ΔΨm, accompanied by inflammation, oxidative stress, apoptosis, and impaired steroidogenesis. Se intervention markedly ameliorated these ultrastructural injuries, preserving mitochondrial morphology and ΔΨm. Functionally, Se suppressed the release of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β); enhanced the activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) while attenuating ROS accumulation; inhibited apoptosis by upregulating

Indexed as

apoptosisGCsinflammationmitochondrial ultrastructureovineoxidative stressseleniumsteroidogenesis

Identifiers

PMID42450802
PMCPMC13359734

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.