ArticleMikrochimica acta2026
Aptamer-based colorimetric assay on poly(allylamine)-blended polycaprolactone electrospun nanofibers for CD36 detection.
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A new bioanalytical system was designed to provide colorimetric determination on nanofibers for the determination of CD36, a biomarker of atherosclerosis. First, electrospun nanofibers (ENFs) with a bead-free morphology and hydrophilic surface were produced using electrospinning with polycaprolactone (PCL) and poly(allylamine) hydrochloride (PAH) polymers. Bioconjugates were prepared using CD36-specific aptamers and gold nanoparticles (AuNPs) for the determination of CD36. Aptamer-AuNPs bioconjugates were incubated with various concentrations of CD36. Then, mixtures of CD36 and Aptamer-AuNPs bioconjugates were applied on NaCl-pretreated PCL-PAH ENFs. Visible color changes occurred on the PCL-PAH ENFs in proportion to the increasing CD36 concentrations. Each color intensity was recorded using a smartphone camera. The linear range of the colorimetric bioassay designed on the PCL-PAH ENFs was found to be 25-400 ng•mL
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