Double-pulse laser capture microdissection with a needle beam for reduced collateral damage in biological samples.

Simo Tian, Zhiguo He, Dongshu Li, Fengqin Shang, Xiangning Li, Hui Gong, Qingming Luo, Xiaoquan Yang

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Article in Biomedical optics express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Simo TianWuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan 430074, China.

Zhiguo HeState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.

Dongshu LiState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.

Fengqin ShangWuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan 430074, China.

Xiangning LiState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.

Hui GongHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou 215123, China.ORCID https://orcid.org/0000-0001-5519-6248

Qingming LuoState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.ORCID https://orcid.org/0000-0002-6725-9311

Xiaoquan YangState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.ORCID https://orcid.org/0000-0003-4865-7864

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Laser capture microdissection (LCM) enables spatially selective isolation of cells and tissues, but precise cutting in thick biological samples is constrained by the limited axial interaction range of Gaussian beams and the side-lobe effects of nondiffractive beams. Here, we propose double-pulse laser capture microdissection with a needle beam (DPLM-NB) for thick biological samples. A 60-μm-long needle beam provides an extended axial interaction range, while double-pulse delay control improves energy localization and cutting selectivity. Numerical simulations show that pre-excitation by the first pulse enhances energy coupling in the main-lobe region and weakens the side-lobe-associated response. In 30-μm-thick frozen mouse brain sections, the method reduces the cutting width from 6.75 to 2.47 μm and the threshold energy density from 5.02 to 1.96 J/cm

Identifiers

PMID42460341

DOI10.1364/BOE.604195

PMCPMC13372365

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.