Evidence map›Paper›PMID 42465397›Full record

ArticlebioRxiv : the preprint server for biology2026

Re-establishment of TAD boundary organization during DNA replication.

Theodore Busby, Adib Keikhosravi, Mohamadreza Fazel, Faisal Almansour, Kathleen S Metz Reed, Laurent Ozbun, Tatiana Karpova, Gianluca Pegoraro, Tom Misteli

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Theodore BusbyCell Biology of Genomes, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Adib KeikhosraviHigh-throughput Imaging Facility, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Mohamadreza FazelOptical Microscopy Core, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Faisal AlmansourCell Biology of Genomes, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Kathleen S Metz ReedCell Biology of Genomes, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID 0000-0003-1466-8258
Laurent OzbunHigh-throughput Imaging Facility, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Tatiana KarpovaOptical Microscopy Core, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Gianluca PegoraroHigh-throughput Imaging Facility, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID 0000-0003-2843-9464
Tom MisteliCell Biology of Genomes, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID 0000-0003-3530-3020

Funding

Nuclear Architecture and DiseaseZIABC010309 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MISTELI, THOMAS A · 2009 to 2025
$39.8M
Epigenetic mechanisms in oncogenic genome organizationFI2GM146623 · NIGMS · NATIONAL CANCER INSTITUTE · PI BUSBY, THEODORE · 2022 to 2022
–
Intramural NIH HHS ZIA BC010309NIGMS NIH HHS FI2 GM146623
6 · The paper itself

Abstract

A ubiquitous feature of higher order genome organization is the presence of topologically associating domains (TADs). The chromatin architectural proteins cohesin and CTCF are known critical organizers of TADs, but the mechanisms of TAD establishment and maintenance, including their accurate duplication during genome replication, are not well characterized. To address this gap, we used high-throughput imaging-based CRISPR/Cas9 knock-out screening to discover chromatin factors involved in maintenance and establishment of TADs. Among the cellular factors that affect TAD organization, we found enrichment for cell cycle proteins, especially components of the DNA replication machinery. Accordingly, we demonstrate that TADs undergo temporary unfolding during S-phase DNA replication and that interference with progression through replication impedes restoration of normal TAD structure. Mechanistically, inhibition of the RPA complex prevents cohesin and CTCF binding, delays post-replication TAD re-folding and affects TAD folding in non-cycling cells. These results provide novel insights into how TAD structures are re-established during genome duplication.

Indexed as

DNA ReplicationGenome OrganizationHigh-Throughput ImagingTopologically Associating Domains (TADs)

Identifiers

PMID42465397
PMCPMC13370505

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.