ReviewProtein engineering, design & selection : PEDS2026
Protein engineering: status report.
Abstract readReview
In one paragraph
Review in Protein engineering, design & selection : PEDS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
81 authors.
Hui-Wang AiCenter for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, United States.
Frances H ArnoldDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Doug BarrickT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, United States.
Hagan BayleyDepartment of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom.
Alfie-Louise R BrownlessSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, United States.
Robert E CampbellDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.ORCID 0000-0003-0604-092X
Roger Castells-GraellsStructural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain.ORCID 0000-0002-3985-6194
Pimchai ChaiyenSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong 21210, Thailand.
Roberto A ChicaDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.ORCID 0000-0003-3789-9841
Lauren O ChisholmDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21218, United States.
Daniel ChristGarvan Institute of Medical Research, Sydney, NSW 2010, Australia.ORCID 0000-0002-7313-3977
Guo-Chao ChuNew Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Michael ChungyounDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, United States.ORCID 0000-0002-8416-5966
Vincent G H EijsinkFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway.ORCID 0000-0002-9220-8743
Michael B ElowitzDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Douglas J FansherDepartment of Chemistry, Université de Montréal, Montréal, QC H2V 0B3, Canada.
Rudi FasanDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080, United States.
Gabriel FoleySamsara Eco, Sydney, NSW 2065, Australia.
Michelle S FreiDepartment of Chemistry and Applied Biosciences, ETH Zürich, Zürich 8093, Switzerland.
Andreas GagsteigerDepartment of Biochemistry, University of Bayreuth, Bayreuth 95447, Germany.ORCID 0000-0003-0112-3400
Ruiyan GaoManufacturing Futures Lab, Department of Chemical Engineering, University College London, London WC1E 7JE, United Kingdom.
Elizabeth M J GillamSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane 4072, Australia.ORCID 0000-0003-0378-793X
Jeffrey J GrayDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, United States.ORCID 0000-0001-6380-2324
Luis F GuerraDepartment of Chemistry, King's College London, London SE1 1DB, United Kingdom.ORCID 0000-0002-8960-1720
Birte HöckerDepartment of Biochemistry, University of Bayreuth, Bayreuth 95447, Germany.ORCID 0000-0002-8250-9462
Zhiyang JinAndrew and Peggy Cherng Department of Medical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Shina Caroline Lynn KamerlinSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, United States.ORCID 0000-0002-3190-1173
Nobuyasu KogaLaboratory for Protein Design, Institute for Protein Research, The University of Osaka, Suita, Osaka 565-0871, Japan.
Roland E KontermannInstitute of Cell Biology and Immunology, University of Stuttgart, Stuttgart 70569, Germany.
Brian KuhlmanDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Olivia A LangloisT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, United States.
Dongyang LiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Roberto D LinsAggeu Magalhaes Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.ORCID 0000-0002-3983-8025
Chang C LiuDepartment of Biomedical Engineering, University of California Irvine, Irvine, CA 92697, United States.
Lei LiuNew Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Diego López BarreiroManufacturing Futures Lab, Department of Chemical Engineering, University College London, London WC1E 7JE, United Kingdom.
Peilong LuNew Cornerstone Science Laboratory, State Key Laboratory of Gene Expression, Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
Sohum MehtaDepartment of Pharmacology, University of California San Diego, La Jolla, CA 92093, United States.
Ikumi MiyazakiDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Manuel M MüllerDepartment of Chemistry, King's College London, London SE1 1DB, United Kingdom.ORCID 0000-0001-6701-0893
Serge MuyldermansLaboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels 1050, Belgium.
Naoto NemotoGraduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan.
Jeff NivalaPaul G. Allen School of Computer Science, Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, United States.
James K NuñezDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, United States.
Izaiah J OrnelasDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, United States.
Joelle N PelletierDepartment of Chemistry, Université de Montréal, Montréal, QC H2V 0B3, Canada.
Andreas PlückthunDepartment of Biochemistry, University of Zürich, Zürich 8057, Switzerland.ORCID 0000-0003-4191-5306
Owen T PorthKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.
Kridsadakorn PrakineeSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong 21210, Thailand.
Yujia QingDepartment of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom.
Julia C ReisenbauerDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Sandy SchmidtDepartment of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Groningen 9713, AV, The Netherlands.
Oliver SeifertInstitute of Cell Biology and Immunology, University of Stuttgart, Stuttgart 70569, Germany.
Mikhail G ShapiroDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Rachna SharmaCenter for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, United States.
Nilmani SinghCarl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana-Champaign, IL 61801, United States.
Benjamin J SmithDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Jamie B SpanglerDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21218, United States.ORCID 0000-0001-8187-3732
Rie Tatsumi-KogaLaboratory for Protein Design, Institute for Protein Research, The University of Osaka, Suita, Osaka 565-0871, Japan.
Takuya TeraiDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Henrik TerholsenDepartment of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Groningen 9713, AV, The Netherlands.ORCID 0000-0001-9625-8177
Mattias TolhurstPaul G. Allen School of Computer Science, Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, United States.
Katherine W TrippT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, United States.
Vanessa Tanja TrossmannDepartment of Biochemistry, University of Bayreuth, Bayreuth 95447, Germany.ORCID 0000-0002-2404-0301
Chandra L TuckerDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, United States.
Onur TurakDepartment of Biochemistry, University of Bayreuth, Bayreuth 95447, Germany.
James A Van DeventerChemical and Biological Engineering Department, Tufts University, Medford, MA 02155, United States.ORCID 0000-0003-4343-6157
Anikó VárnaiFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway.ORCID 0000-0002-2796-514X
Isabelle F T VianaDepartment of Fundamental Chemistry and Materials Science Graduate Program, Federal University of Pernambuco, Recife 50670-901, Brazil.ORCID 0000-0003-4648-6635
Amy M WeeksDepartments of Biochemistry and Chemistry, University of Wisconsin-Madison, Madison, WI 53705, United States.
K Dane WittrupKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.
Kingsley L-J WongPaul G. Allen School of Computer Science, Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, United States.
Chenggang XiDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, United States.
Todd O YeatesDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, United States.ORCID 0000-0001-5709-9839
Andy Hsien-Wei YehDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, United States.
Jin ZhangDepartment of Pharmacology, University of California San Diego, La Jolla, CA 92093, United States.
Tiger S ZhangDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.ORCID 0000-0002-6669-8814
Huimin ZhaoCarl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana-Champaign, IL 61801, United States.
Fang ZhengDepartment of Biochemistry and Molecular Biology, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
Jingyi ZhuNew Cornerstone Science Laboratory, State Key Laboratory of Gene Expression, Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.ORCID 0000-0002-0022-3744
Sacha ZinnGarvan Institute of Medical Research, Sydney, NSW 2010, Australia.
Funding
Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2MPrediction of the Structures of Protein ComplexesR35GM141881 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI JEFFREY J GRAY · 2021 to 2026
$7.6MMechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6MREPEAT-PROTEINS;STABILITY, FOLDING KINETICS &EVOLUTIONR01GM068462 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI BARRICK, DOUGLAS E. · 2005 to 2025
$6.3MGPU workstation for deep learning-based protein design and cryo-EM data processingR35GM131923 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BRIAN A KUHLMAN · 2019 to 2026
$6.1MA Scalable Platform to Discover Antimicrobials of Ribosomal OriginR01AI144967 · NIAID · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Douglas Alan Mitchell, WILFRED A. VAN DER DONK · 2019 to 2026
$6.0MNext-Gen Fluorescent Indicators for Imaging Synaptic Zinc in Cortical Sound ProcessingR01EB033172 · NIBIB · UNIVERSITY OF VIRGINIA · PI Huiwang Ai, Thanos Tzounopoulos · 2022 to 2026
$3.3MUltrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3MThe yeast surface as a platform for inhibitor discoveryR35GM133471 · NIGMS · TUFTS UNIVERSITY MEDFORD · PI James Allen Van Deventer · 2019 to 2026
$3.1MUncovering new approaches to manipulate and probe biochemical processes with spatial and temporal precision.R35GM136367 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Chandra L Tucker · 2020 to 2026
$3.0MAMPK Regulation of ACE2 in Endothelial Health and DiseaseR01HL162302 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHYY, JOHN YJ, ZHANG, JIN · 2022 to 2025
$2.7MOptimization of CaMPARI for large-scale, cellular-resolution activity recording in freely-moving miceU01NS123658 · NINDS · CLEVELAND CLINIC LERNER COM-CWRU · PI CAMPBELL, ROBERT E., DANA, HOD MICHAEL · 2021 to 2025
$2.6MNCI NIH HHS R01 CA262815NCI NIH HHS R35 CA197622NHLBI NIH HHS R01 HL162302NIAID NIH HHS R01 AI144967NIA NIH HHS R01 AG077773NIBIB NIH HHS R00 EB031913NIBIB NIH HHS R01 EB033172NIBIB NIH HHS R01 EB035430NIDCR NIH HHS R01 DE030497NIDDK NIH HHS R01 DK073368NIGMS NIH HHS DP2 GM149548NIGMS NIH HHS R01 GM068462NIGMS NIH HHS R35 GM131923NIGMS NIH HHS R35 GM133471NIGMS NIH HHS R35 GM136367NIGMS NIH HHS R35 GM141881NIGMS NIH HHS R35 GM155044NIGMS NIH HHS R35 GM158123NIMH NIH HHS RF1 MH126707NINDS NIH HHS U01 NS123658Wellcome Trust
Abstract
With this status report, we aim to provide a timely snapshot of the protein engineering field as a broad and rapidly advancing discipline that integrates computational, molecular biology, structure-guided, evolutionary, and synthetic approaches to create new and improved proteins with tailored structures and useful functions. The report is organized into eight thematic areas spanning core methodologies and major application domains, including enzymes, therapeutics, detection, synthetic biology, and materials. Contributions from experts across these areas highlight both the historical foundations and recent advances in their respective fields, with particular emphasis on the growing influence of machine learning and artificial intelligence-based methods. Emerging from this broad overview is a central message: protein engineering appears to be entering a golden age, defined by a rapidly accelerating pace of progress, even as significant challenges in design, screening, and real-world application remain. Looking ahead, the continued integration of computational and experimental strategies is poised to further accelerate the impact of protein engineering across an expanding range of economically and societally important sectors, from therapeutics and molecular imaging to diagnostics, plastic recycling, and industrial chemistry.
Indexed as
Protein EngineeringProteinsComputational BiologyHumansSynthetic BiologyProteinsapplication areascomputational methodsexperimental techniques
Identifiers
What Socratic holds
Textmetadata
LicenceCC BY
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.