Wheat Doubled Haploid Production Through Maize Crossing.

Doubled haploid (DH) technology is a powerful tool in cereal breeding, enabling the rapid production of homozygous lines from heterozygous plants. This technique accelerates the development of new cereal varieties by producing genetically uniform plants in just one generation, a process that would otherwise require several generations of self-pollination. DH methods are particularly valuable in breeding programs for wheat, barley, maize, and rice, where they enhance selection efficiency and shorten the breeding cycle. The process typically involves inducing haploidy through interspecies hybridization, using inducer genotypes, anther or microspore culture, followed by chromosome doubling to restore fertility. The resulting DHs are genetically stable and carry fixed traits, making them ideal for genetic studies, trait selection, and commercial cultivation. In cereals, commonly used methods include wide hybridization, androgenesis, and gynogenesis. Wide hybridization, such as wheat-maize crosses, is one of the most frequently used approaches to producing haploids, while treatments like colchicine are used for chromosome doubling to produce fertile, DH plants. By combining these techniques, breeders can rapidly develop high-yielding, disease-resistant, and stress-tolerant cereal varieties tailored to the demands of modern agriculture.