Evidence map›Paper›PMID 42472233›Full record

ArticleJournal of pharmaceutical analysis2026

Self-assembled multifunctional hairpin probe for ultrasensitive and mismatch-selective miRNA detection.

Baoqiang Chen, Yiping Fan, Qi Wang, Jianguo Xu, Yi Wang, Bingyong Lin, Lee Jia, Longhua Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Baoqiang ChenProvincial Key Laboratory of Multimodal Perceiving and Intelligent Systems, Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.
Yiping FanKey Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Infectious Diseases in the Elderly in Zhejiang Province (Cultivation), Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University, Jiaxing, Zhejiang, 314000, China.
Qi WangSchool of Biological and Food Engineering, Fuyang Normal University, Fuyang, Anhui, 236037, China.
Jianguo XuProvincial Key Laboratory of Multimodal Perceiving and Intelligent Systems, Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.
Yi WangDepartment of Oncology, Hefei First People's Hospital, Third Affiliated Hospital of Anhui Medical University, Hefei, 230032, China.
Bingyong LinProvincial Key Laboratory of Multimodal Perceiving and Intelligent Systems, Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.
Lee JiaChina-Ireland Food & Medicine Innovation Joint Laboratory, The First Affiliated Hospital, Henan University, Kaifeng, Henan, 475004, China.
Longhua GuoProvincial Key Laboratory of Multimodal Perceiving and Intelligent Systems, Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sensitive, specific, and stable detection of microRNAs (miRNAs) in complex biological environments remains a formidable challenge in molecular diagnostics. We introduce a novel bidirectional palindromic assembled multifunctional hairpin probe (A-MF-HP)-a molecular tool that integrates target recognition, cascade signal amplification, and fluorescence reporting into a single, compact system. Two palindromic arms drive autonomous, bidirectional self-assembly into a nuclease-resistant architecture, enabling robust operation in serum-rich environments. Upon recognition of miRNA-21, a structural switch in one hairpin triggers polymerase extension, nicking, and strand displacement all without auxiliary probes. This initiates a self-propagating disassembly cascade, unfolding additional hairpins and exponentially amplifying the signal. Under the optimized conditions, the platform demonstrates femtomolar-level sensitivity (about 1× 10

Indexed as

BiostabilityDNA assemblyIsothermal amplificationmiRNA analysisMultifunctional hairpin probePalindromic structure

Identifiers

PMID42472233
PMCPMC13380003

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.