Evidence map›Paper›PMID 42478773›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Super-Multiplexed Label-Free Raman Imaging Uncovers Novel Testicular Metabolic Couplings for Residual Body and Spermatogonial Differentiation.

Jiaxin Li, Xia Chen, Yujuan Qi, Chen Qiao, Qiuru Huang, Dan Zhao, Weiyi Qian, Li Di, Yi Shi, Xiangqi Hu and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Jiaxin LiClinical Research Center for Reproductive Genetics, Center for Reproductive Medicine, Affiliated Maternity and Child Health Care Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Xia ChenCenter for Reproductive Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Yujuan QiDepartment of Reproductive Medicine, Xuzhou Central Hospital, Xuzhou Central Hospital affiliated to Southeast University, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China.
Chen QiaoDepartment of Clinical Pharmacy, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.ORCID https://orcid.org/0000-0003-4315-0351
Qiuru HuangInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Dan ZhaoReproductive Medicine Center, Reproductive Sciences Institute, The Fourth Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
Weiyi QianInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Li DiInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Yi ShiInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Xiangqi HuDepartment of Clinical Pharmacy, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
Yueyue ShaoChangzhou Medical Center, Changzhou Maternal and Child Health Care Hospital, Nanjing Medical University, Changzhou, China.
Kehan WangState Key Laboratory of Reproductive Medicine and Offspring Health, Center For Reproduction and Genetics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, China.
Zhiyu Tang-YangInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Hanzhi ZangInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Yuwen DuInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Anran DongInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Jinxiong FengInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Jiayun ChenInstitute of Reproductive Medicine, Medical School, Nantong University, Nantong, China.
Ying ZhengDepartment of Histology & Embryology, Faculty of Medicine, Yangzhou University, Yangzhou, China.
Xiaofang TanClinical Research Center for Reproductive Genetics, Center for Reproductive Medicine, Affiliated Maternity and Child Health Care Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Xiaoyu ZhaoSuperVision Medicine Co,.Ltd, Wuxi, China.
Zhonghua ShiChangzhou Medical Center, Changzhou Maternal and Child Health Care Hospital, Nanjing Medical University, Changzhou, China.ORCID https://orcid.org/0000-0001-8728-9158
Bo ZhengState Key Laboratory of Reproductive Medicine and Offspring Health, Center For Reproduction and Genetics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, China.ORCID https://orcid.org/0000-0002-1496-0753
Jun YuClinical Research Center for Reproductive Genetics, Center for Reproductive Medicine, Affiliated Maternity and Child Health Care Hospital of Nantong University, Medical School of Nantong University, Nantong, China.

Funding

Basic Research Foundation of Zhenjiang JC2024024Basic Research Foundation of Zhenjiang JC2024039Medical Research Project of Jiangsu Provincial Health Commission M2022071National Natural Science Foundation of China 32470899National Natural Science Foundation of China 82271633National Natural Science Foundation of China 82301812National Natural Science Foundation of China 82371697National Natural Science Foundation of China 82471641Noncommunicable Chronic Diseases-National Science and Technology Major Project 2024ZD0532100Noncommunicable Chronic Diseases-National Science and Technology Major Project 2024ZD0532103Science Foundation of Nantong Science and Technology Plan MS2024066Xuzhou City's Frontier Technology Research and Development Program KC25076Xuzhou Pengcheng Talents-Medical young reserve talents XWRCH20220011
6 · The paper itself

Abstract

The biochemical composition underlying cytoplasmic residual body (RB) and arrested spermatogonia remains unresolved in spermatogenesis, in part due to limited approaches for intact-tissue metabolic interrogation. Here we develop Super-multiplexed Label-free Raman Imaging (SLRI) to generate 2D/3D biochemical maps of intact Drosophila testes. SLRI delineates major testicular cell types and provides the first in situ, stage-resolved mapping of cytoplasmic RBs at two discrete phases. We further identify a differentiation-arrest-associated metabolic trajectory in spermatogonial clusters, characterized by coordinated spatiotemporal decay of specific biochemical species. Across 42 spatiotemporally variant metabolites and metabolic pathways, SLRI reveals RB- and arrest-enriched signatures. Notably, RBs and arrested spermatogonia display significant biochemical overlap, alongside unique spectral signatures. Furthermore, we uncovered a ring-like, punctate arrangement of lipids, amino acids, and vitamins encircling RBs, suggesting structured metabolite partitioning during cytoplasmic remodeling. Collectively, SLRI establishes a general framework for high-dimensional metabolic mapping and remodeling analysis in intact tissues.

Indexed as

label‐freemetabolic remodelingraman imagingresidual bodyspermatogonial differentiation

Identifiers

PMID42478773
PMCPMC13387037

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.