ArticleFrontiers in plant science2026
Integration of widely targeted and targeted metabolomics reveals flavonoid accumulation profiles across different cultivars of
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Methods: We employed an integrated approach combining widely targeted and targeted metabolomics data to systematically elucidate the metabolic profile differences among five major Results: A total of 2,286 metabolites were identified, from which 802 differential metabolites were identified, with the majority belonging to flavonoids, lipids, and terpenoids. Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) revealed that the metabolic profiles of the XGH and YL cultivars were significantly distinct from the others. KEGG pathway enrichment analysis indicated that phenylpropanoid biosynthesis, flavonoid biosynthesis, and flavone and flavonol biosynthesis are the core pathways driving cultivar differentiation. Additionally, three common differential Kaempferol-4'-O-glucoside*, 1-O-(3,4,5-Trimethoxybenzoyl)-β-D-Glucopyranoside, and sec-o-Glucosylhamaudol were identified as potential key chemical markers for cultivar discrimination. Targeted quantitative analysis further validated that XGH and YL possess a significant advantage in the accumulation of 11 key flavonoid components, with flavonol glycosides exhibiting the most prominent accumulation characteristics. Discussion: This study represents the first system-level investigation into the metabolic diversity of
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