Evidence map›Paper›PMID 42487119›Full record

ArticleBMC veterinary research2026

Neutralizing nanobodies against porcine epidemic diarrhea virus: discovery and characterization.

Mengya Fan, Xuran Wang, Yangyang Yan, Lili Wang, Jiayi Wei, Guohu Chen, Yufei Wang, Junlan Zan, Lianping Xu, Fengsai Li and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Mengya Fan *Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Xuran Wang *Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Yangyang Yan *Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Lili WangHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Jiayi WeiHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Guohu ChenHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Yufei WangHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Junlan ZanBayingol Mongolian Autonomous Animal Disease Control and Diagnosis Center, Kurle, 841000, China.
Lianping XuBayingol Mongolian Autonomous Animal Disease Control and Diagnosis Center, Kurle, 841000, China.
Fengsai LiHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Shijie YanHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Shanshan YangHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Ping RuiHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Zengjun MaHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Wei LiuKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430070, China. liuwei@hbaas.com.
Tao SongHebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China. songtaoer@126.com.

Funding

he earmarked fund for Hebei Agriculture Research System (HARS), China HBCT2024220208open project of Key laboratory of prevention and control agents for animal bacteriosis (Ministry of Agriculture and Rural Affairs) KLPCAAB-2025-04S&T Program of Hebei, China 21324801D
6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) causes severe mortality in piglets, inflicting significant economic losses globally. Nanobodies (Nbs) offer distinct advantages over conventional antibodies for disease intervention. This study aimed to develop neutralizing Nbs against the PEDV spike (S) protein. We constructed a eukaryotic plasmid encoding the S protein of the CH-HB2-2018 strain and expressed it in HEK-293 F cells. Following alpaca immunization with the purified S protein, a phage display library was constructed. Biopanning yielded 70 unique Nbs based on CDR diversity, with 67 confirmed as specific binders via ELISA and Western blot. Further characterization using immunofluorescence and flow cytometry identified 9 specific candidates. Microneutralization assays revealed that these candidates showed varying degrees of inhibition against PEDV replication in Vero cells. Notably, nanobody 3Nb17 exhibited the highest binding affinity and neutralizing titer. These findings highlight 3Nb17 as a promising candidate for developing novel PEDV diagnostic tools and therapeutics.

Indexed as

Antibodies, NeutralizingAntibodies, ViralCoronavirus InfectionsPorcine epidemic diarrhea virusSingle-Domain AntibodiesAnimalsCamelids, New WorldChlorocebus aethiopsHEK293 CellsHumansNeutralization TestsSpike Glycoprotein, CoronavirusSwineSwine DiseasesVero CellsAntibodies, NeutralizingAntibodies, ViralSingle-Domain AntibodiesSpike Glycoprotein, CoronavirusNanobodyNeutralizing antibodyPhage displayPorcine epidemic diarrhea virusSpike protein

Identifiers

PMID42487119
PMCPMC13393878

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.