Evidence mapPaperPMID 42487140Full record

ArticleChinese medicine2026

Yiyi Fuzi Baijiang formula protects against DSS-induced colitis by orchestrating the gut barrier-microbiota-metabolism axis.

Yin Bao, Qiang Ao, Meng Wang, Xiaoling Mao, Jun Zhu, Mingyue Zhang, Yang Chen, Hong Zhu, Jun Gao

Abstract read
In one paragraph

Article in Chinese medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yin Bao *Department of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Qiang Ao *Department of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Meng Wang *Department of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Xiaoling MaoDepartment of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Jun ZhuDepartment of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Mingyue ZhangDepartment of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China.
Yang ChenState Key Laboratory of Phytochemistry and Natural Medicines, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Hong Zhu *Department of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China. 24zhuhong@163.com.
Jun Gao *Department of Gynecologic Oncology, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College Nanchang, Nanchang, 330036, China. gaojun@ncmc.edu.cn.

Funding

"Five-Level Progressive" Talent Cultivation Project of Jiangxi Cancer Hospital & Institute, Jiangxi Cancer Hospital (the Second Affiliated Hospital of Nanchang Medical College) Talent Training Support Plan WCDJ2024XK01Nanchang Medical College Discipline Leader Project: the Clinical Medicine of Integrated Traditional Chinese and Western Medicine Gynecology and PediatricsNational Natural Science Foundation of China 82060477Natural Science Foundation of Jiangxi Province 20202BAB206054Natural Science Foundation of Jiangxi Province 20242BAB25525Science and Technology Plan Project of Jiangxi Provincial Administration of Traditional Chinese Medicine 2024A0092Science and Technology Plan Project of Jiangxi Provincial Administration of Traditional Chinese Medicine 2024B0158Science and Technology Project of Jiangxi Provincial Health Commission 2025L3049Science and Technology Project of Jiangxi Provincial Health Commission 2025L3050Teaching Reform Research Project of Nanchang Medical College NYJG-20250050Teaching Reform Research Project of Nanchang Medical College NYJG-20250055
6 · The paper itself

Abstract

backgroundInflammatory bowel disease (IBD) is a relapsing inflammatory disorder of the gastrointestinal tract with increasing global incidence. Current therapies are often limited by side effects, loss of efficacy, and high cost, underscoring the need for safer and more effective alternatives, particularly multi-target agents derived from natural products. PURPOSE: This study aimed to elucidate the protective mechanisms of Yiyi Fuzi Baijiang formula (YFB), a traditional Chinese medicine (TCM) formulation, against dextran sulfate sodium (DSS)-induced acute colitis, focusing on its systemic regulation of the gut barrier-microbiota-metabolism axis.

methodsWe employed an integrated approach combining network pharmacology, UPLC-Q-TOF-MS/MS-based phytochemical analysis, in vivo evaluation in a DSS-induced colitis mouse model, 16S rRNA gene sequencing, and untargeted metabolomics to assess the effects of YFB and uncover its mechanisms of action.

resultsNetwork pharmacology predicted, and experiments confirmed, that core YFB components (e.g., quercetin, kaempferol) act via IL-17, TNF, and NF-κB pathways. YFB administration dose-dependently improved disease activity index, colon shortening, and histopathology in colitis mice. It restored intestinal barrier integrity by upregulating ZO-1, Occludin, and MUC2, while suppressing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, IL-17A) and NF-κB activation. Critically, YFB promoted epithelial repair by restoring the expression of intestinal stem cell marker LGR5 and progenitor cell marker SOX9, and by normalizing the aberrant increase in endocrine cell marker CHGA. YFB treatment was associated with reversal of DSS-induced gut microbiota dysbiosis, restoration of diversity, enrichment of beneficial bacteria (e.g., Lachnospiraceae), and suppression of opportunistic pathogens (e.g., Enterobacteriaceae). Untargeted metabolomics showed that YFB treatment was associated with modulation of DSS-altered fecal metabolites (e.g., fatty acids, bile acids) and pathways such as "microbial metabolism in diverse environments".

conclusionYFB, when administered concomitantly with DSS, protects against DSS-induced colitis via the synergistic effects of its multi-component system. Its mechanism entails systemic regulation of the gut barrier-microbiota-metabolism axis, involving suppression of NF-κB-driven inflammation, promotion of intestinal epithelial repair (via LGR5/SOX9/CHGA modulation), restoration of the intestinal barrier, alterations in gut microbiota, and modulation of host-microbial co-metabolism. These findings provide a scientific basis for YFB's clinical application and highlight the value of TCM formulations in managing complex multi-factorial diseases.

Indexed as

ColitisGut microbiotaInflammatory bowel diseaseMetabolomicsNetwork pharmacologyYiyi Fuzi Baijiang formula

Identifiers

PMID42487140
PMCPMC13393498

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.