Evidence map›Paper›PMID 42490019›Full record

ReviewStem cell reviews and reports2026

Optimized Metabolic Conditions Reveal the Adipogenic Competence of Wharton's Jelly MSCs: Insight from Literature Review and Experimental Validation.

Cécile Pinault, Audrey Cras, Michel-Gabriel Cazenave, Anne Schnitzler, Jeanne Rancic, Jerome Larghero, Sophie Vacher, Curtis L Cetrulo, Daniel Balvay, Ivan Bieche and 2 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Stem cell reviews and reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Cécile PinaultParis Cité University, INSERM PARCC, Paris, 75015, France.
Audrey CrasAP-HP, Cell therapy department, INSERM Centre d'Investigation Clinique en Biothérapies CIC-BT, Saint-Louis Hospital, Paris, 75010, France.
Michel-Gabriel CazenaveParis Cité University, INSERM PARCC, Paris, 75015, France.
Anne SchnitzlerDepartment of Genetics, Institut Curie, Paris, France.
Jeanne RancicParis Cité University, INSERM PARCC, Paris, 75015, France.
Jerome LargheroAP-HP, Cell therapy department, INSERM Centre d'Investigation Clinique en Biothérapies CIC-BT, Saint-Louis Hospital, Paris, 75010, France.
Sophie VacherDepartment of Genetics, Institut Curie, Paris, France.
Curtis L CetruloDivision of Plastic and Reconstructive Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.
Daniel BalvayParis Cité University, INSERM PARCC, Paris, 75015, France.
Ivan BiecheDepartment of Genetics, Institut Curie, Paris, France.
Alexandre G LellouchParis Cité University, INSERM PARCC, Paris, 75015, France.
David M SmadjaParis Cité University, INSERM PARCC, Paris, 75015, France. david.smadja@aphp.fr.ORCID http://orcid.org/0000-0001-7731-9202

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stromal/stem cells (MSCs) derived from distinct tissue niches exhibit marked functional heterogeneity, yet the determinants governing their adipogenic competence remain incompletely understood. Wharton's jelly MSCs (WJ-MSCs) are frequently considered poorly adipogenic compared with bone marrow MSCs (BM-MSCs), although the contribution of culture conditions to this phenotype has not been systematically investigated. Here, integrating a structured review of 41 studies with experimental modeling, we demonstrate that adipogenic commitment of WJ-MSCs is highly dependent on metabolic induction conditions and exposure duration. Structured literature review revealed substantial heterogeneity in adipogenic protocols across the literature, with no consensus framework for WJ-MSCs differentiation. Guided by these observations, we compared four adipogenic conditions differing in glucose availability and insulin supplementation and quantified differentiation using machine learning-assisted lipid imaging. Whereas BM-MSCs readily underwent adipogenesis under conventional induction conditions, WJ-MSCs displayed delayed and limited differentiation. However, prolonged induction combined with enhanced metabolic support revealed a delayed but significant adipogenic responsiveness for WJ-MSCs. High glucose and insulin synergistically promoted lipid accumulation in both MSCs sources, with the strongest effects observed under combined conditions. These findings identify metabolic context as a critical regulator of MSCs lineage plasticity and establish a standardized framework for evaluating adipogenic differentiation across tissue-derived MSCs populations.

Indexed as

AdipogenesisMesenchymal Stem CellsWharton JellyAnimalsCell DifferentiationGlucoseHumansInsulinGlucoseInsulinAdipogenic differentiationBone marrowMetabolic regulationMSCsWharton’s jelly

Identifiers

PMID42490019
PMCPMC13550029

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.