Evidence map›Paper›PMID 42496326›Full record

ArticleNeuroSci2026

Effect of Caffeine on Cell Death, Oxidative Stress, and Microglial Morphology in a Ferret Organotypic Brain Slice Model of Hypoxia-Ischemia.

Olivia C Brandon, Kylie A Corry, Zheyu Ruby Jin, Kate F DiNucci, Matthew J Magoon, Nels Schimek, Daniel H Moralejo, Sandra E Juul, Patrick M Boyle, Elizabeth A Nance and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NeuroSci, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Olivia C BrandonDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0009-0752-9509
Kylie A CorryDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-2796-2184
Zheyu Ruby JinDepartment of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0008-6022-6809
Kate F DiNucciDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0003-6217-0283
Matthew J MagoonDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-5947-3772
Nels SchimekDepartment of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Daniel H MoralejoDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.
Sandra E JuulDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-6861-6229
Patrick M BoyleDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Elizabeth A NanceDepartment of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0001-7167-7068
Thomas R WoodDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-1130-2720
Sarah E KolnikDivision of Neonatology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0003-1397-8430

Funding

University of Washington NBRIF
6 · The paper itself

Abstract

Brain injury after hypoxia-ischemia (HI) is the leading cause of morbidity and mortality in term and near-term neonates worldwide. The ferret is a promising translational model to study HI due to its gyrified brain and white-to-gray matter ratio that more closely resembles humans compared to rodents. Caffeine, an adenosine A2A receptor (A2AR) antagonist, shows neuroprotective potential after HI, but its effects have not been fully characterized. We sought to evaluate caffeine's effect on neuronal cell death, cytotoxicity, and inflammatory and oxidative stress markers in a term-equivalent ferret organotypic brain slice model of HI. Slices were cultured for 72 h, exposed to two hours of oxygen-glucose deprivation (OGD), and randomized to OGD alone, OGD with caffeine (20 or 50 mg/L), or OGD with caffeine and an A2AR agonist. Healthy slices served as controls. Outcomes included global cell death, regional cell death, microglial morphology, and expression of inflammatory and oxidative stress genes (46-48 slices/group for cell death assays and 18 slices/group for imaging, balanced by sex). Caffeine 50 mg/L significantly reduced global cell death compared to OGD (

Indexed as

caffeineferrethypoxia–ischemianeonatalorganotypic slice culture

Identifiers

PMID42496326
PMCPMC13398045

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.