ArticleAnalytical and bioanalytical chemistry2026
Validated SPE-HPLC-MS/MS analysis of steroid hormones in H295R cell culture medium: chromatographic separation and ESI response in the presence of potential endocrine-disrupting chemicals.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A sensitive and robust SPE-HPLC-MS/MS method was developed and validated for the simultaneous quantification of five steroid hormones in H295R cell culture medium. Chromatographic separation of the target steroids and selected potential endocrine‑disrupting chemicals (EDCs) was achieved using a ZORBAX Eclipse Plus C18 column (1.8 µm, 95 Å, 50 × 2.1 mm). Sample preparation was performed by solid‑phase extraction employing hydrophilic-lipophilic balanced polymeric reversed‑phase sorbent cartridges. Extraction recovery was evaluated at three concentration levels, tailored to each steroid, and exceeded 80% for all analytes. Intra‑ and inter‑day precision, expressed as relative standard deviation (RSD), ranged from 1 to 12%. Method limits of quantification were in the range of 8 and 73 pg mL⁻
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