Evidence map›Paper›PMID 42497058›Full record

ArticleSTAR protocols2026

Protocol for purifying iPSC-derived retinal pigment epithelial cells by sequential digestion and intrinsic autofluorescence sorting.

Fengming Yang, Rui Li, Xinrun Lv, Boling Chu, Jiuhua Luo, Rongjing Ge, Yongjun Luo, Laibin Ren, Bei Liu, Jing Hu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Fengming YangSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China. Electronic address: yangfengming01@outlook.com.
Rui LiSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Xinrun LvSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Boling ChuSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Jiuhua LuoThe First College of Clinical Medical Science, China Three Gorges University, Yichang 443000, Hubei, China; Department of Ophthalmology, Yichang Central People's Hospital, Yichang 443000, Hubei, China.
Rongjing GeDepartment of Pathophysiology, Bengbu Medical College, Bengbu 233000, Anhui, China.
Yongjun LuoChengdu Yunce Medical Biotechnology Co., Ltd., Chengdu 610054, Sichuan, China.
Laibin RenChengdu Yunce Medical Biotechnology Co., Ltd., Chengdu 610054, Sichuan, China.
Bei LiuSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Jing HuSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Haijiang ZhangThe First College of Clinical Medical Science, China Three Gorges University, Yichang 443000, Hubei, China; Department of Ophthalmology, Yichang Central People's Hospital, Yichang 443000, Hubei, China. Electronic address: ycszxrmyy1@ctgu.edu.cn.
Hao QianSchool of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China. Electronic address: qianhao@uestc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSC)-derived retinal pigment epithelium cells serve as a promising cell source for transplantation therapy in age-related macular degeneration. Here, we present a protocol for purifying the mature retinal pigment epithelium (RPE) cells post-differentiation. We describe steps for sequentially digesting cells with different enzymes and sorting them based on the autofluorescence of mature RPE cells, avoiding fixation and immunostaining. By maintaining high cell viability and purity, this cost-efficient protocol has broad implications for both in vitro research and transplantation applications.

Indexed as

Cell DifferentiationCell separation/fractionationFlow CytometryStem Cells

Identifiers

PMID42497058
PMCPMC13427439

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.