Evidence map›Paper›PMID 42498788›Full record

ArticleAnalytical and bioanalytical chemistry2026

Plasma LC-HRMS metabolomics in parkinsonian syndromes: comparative evaluation of sample preparation protocols for exploratory metabolic signatures.

Erika Esposito, Alessandro Perrone, Giovanna Lopane, Rosalba Vitagliano, Nicolò Interino, Alice Caravelli, Chiara Cancellerini, Giovanna Calandra-Buonaura, Jessica Fiori, Manuela Contin

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Erika EspositoIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy.
Alessandro PerroneCentre for Applied Biomedical Research (CRBA), University of Bologna, Bologna, Italy.
Giovanna LopaneIRCCS Istituto Delle Scienze Neurologiche di Bologna, Unit of Rehabilitation Medicine, Bologna, Italy.
Rosalba VitaglianoIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy.
Nicolò InterinoIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy.
Alice CaravelliIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy.
Chiara CancelleriniDepartment of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Giovanna Calandra-BuonauraDepartment of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Jessica FioriIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy. jessica.fiori@unibo.it.ORCID http://orcid.org/0000-0002-6004-5118
Manuela ContinIRCCS, Istituto Delle Scienze Neurologiche di Bologna, Laboratorio di Proteomica Metabolomica e Chimica Bioanalitica, Bologna, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Untargeted plasma metabolomics by LC-HRMS is increasingly used to explore candidate biomarkers in neurodegenerative disease, yet the analytical output and downstream biological interpretability remain highly dependent on end-to-end workflow choices. This limits reproducibility and cross-study comparability in parkinsonian syndromes where objective fluid biomarkers for differential diagnosis are still lacking. Here, we implemented a QC-anchored, untargeted LC-HRMS plasma platform to compare sample preparation strategies and to explore disease-associated metabolic patterns across Parkinson's disease (PD), multiple system atrophy-parkinsonian subtype (MSA-P), and progressive supranuclear palsy-parkinsonism (PSP-P) in a prospective single-center cohort (n = 102; 57 PD, 19 MSA-P, 26 PSP-P). Three protein precipitation-based workflows were compared within a harmonized analytical framework: tube precipitation, 96-well precipitation-filtration, and 96-well phospholipid removal. Integrated preparation QCs, system QCs, and blanks supported performance monitoring, while MS

Indexed as

LC-HRMSMetabolomicsParkinsonian syndromesSample preparation

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.