Evidence map›Paper›PMID 42503045›Full record

ArticleChemphyschem : a European journal of chemical physics and physical chemistry2026

Complexation of Hen Egg White Lysozyme With Bioactive Phenolic Acids: Molecular Insights Into the Binding Mechanisms and Inhibitory Effects Against Protein Aggregation.

Sona Lyndem, Sibasree Hojaisa, Roohi Choudhury, Sadia Nudrat, Kalpana Kumari, Anupam Nath Jha, Atanu Singha Roy

Abstract read
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In one paragraph

Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sona LyndemDepartment of Chemical and Biological Sciences, National Institute of Technology Meghalaya, Sohra, Meghalaya, India.
Sibasree HojaisaDepartment of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India.
Roohi ChoudhuryDepartment of Chemical and Biological Sciences, National Institute of Technology Meghalaya, Sohra, Meghalaya, India.
Sadia NudratDepartment of Chemical and Biological Sciences, National Institute of Technology Meghalaya, Sohra, Meghalaya, India.
Kalpana KumariDepartment of Bioscience and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Anupam Nath JhaDepartment of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India.
Atanu Singha RoyDepartment of Chemical and Biological Sciences, National Institute of Technology Meghalaya, Sohra, Meghalaya, India.ORCID 0000-0003-1902-403X

Funding

Council of Scientific and Industrial Research, India 01/2903/17/ EMR-II
6 · The paper itself

Abstract

Protein misfolding and aggregation are central to many protein aggregation disorders, highlighting the need for effective aggregation inhibitors. This study investigated fructose-induced aggregation of hen egg-white lysozyme (HEWL) and its modulation by the phenolic acids ferulic acid (FA), caffeic acid (CA) and chlorogenic acid (CGA) at 100 and 200 μM. Circular dichroism and ThT fluorescence assays showed that fructose promoted aggregation through increased β-sheet content and fluorescence intensity, respectively, whereas all phenolic acids suppressed β-sheet formation. CA displayed the highest aggregation inhibition activity, followed by FA and CGA. FESEM imaging confirmed fructose-induced HEWL aggregation without fibril formation and demonstrated disruption of aggregates in the presence of phenolic acids. These findings were explained by other biophysical characterisation studies. Fluorescence spectroscopy indicated a static quenching mechanism for all complexes, with FA and CA displaying atypical quenching behaviour. Binding constants suggested moderate affinity, with CA (4.11 ± 0.57 × 10

Indexed as

HydroxybenzoatesMuramidaseAnimalsCaffeic AcidsChickensChlorogenic AcidCoumaric AcidsProtein AggregatesProtein BindingSpectrometry, Fluorescencecaffeic acidCaffeic AcidsChlorogenic AcidCoumaric Acidsferulic acidhen egg lysozymeHydroxybenzoatesMuramidasephenolic acidProtein Aggregatesaggregationdockingfluorescenceglycationlysozymephenolic acids

Identifiers

PMID42503045
PMCPMC13401913

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.