Evidence map›Paper›PMID 42507049›Full record

ArticleMolecular biology reports2026

Muscle invasion in bladder cancer is associated with increased expression of ATG5, LC3A and CHOP.

Maha Ali, Shimaa E Soliman, Thoraya S Eldeeb, Ebrahim Aboeleuon, Shimaa H Shaban, Dalia G Mahran, M F Abdelhafez, Shaimaa R Ata, Hesham S Ata, Eman Radwan

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Maha AliDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Shimaa E SolimanDepartment of Pathology, College of Medicine, Qassim University, Qassim, 51452, Saudi Arabia. S.saad@qu.edu.sa.ORCID http://orcid.org/0000-0003-3919-3818
Thoraya S EldeebDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Ebrahim AboeleuonSurgical Oncology Department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Shimaa H ShabanOncologic Pathology Department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Dalia G MahranDepartment of Public Health and Community Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt.
M F AbdelhafezDepartment of Urology, Urology and Nephrology Hospital, Faculty of Medicine, Assiut University, Assiut, Egypt.
Shaimaa R AtaDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Hesham S AtaDepartment of Pathology, College of Medicine, Qassim University, Qassim, 51452, Saudi Arabia.
Eman RadwanDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Funding

Assiut University 2017/08/27-004-R1
6 · The paper itself

Abstract

backgroundBladder cancer (BC) is considered one of the most prevalent malignant cancers of the urinary system. Recently, autophagy was found to be involved in tumor development.

objectivesInvestigating the link between BC and autophagy, highlighting the role of ER stress in tumor growth and invasiveness.

methodsSixty urothelial carcinoma patients recruited to this study were divided into 2 groups: Low-grade BC (LGBC) and High-grade BC (HGBC), which were further classified as muscle-invasive (HGMI) or non-muscle-invasive (HGNMI). Control tissue samples were collected from the safety margin. Levels of ATG5 and caspase 3 were determined using qRT-PCR; CHOP levels by ELISA; and malondialdehyde using the lipid peroxide assay kit. LC3A expression was detected by immunohistochemistry.

resultsSignificant upregulation of tissue levels of ATG5, CHOP, MDA and LC3A was observed in tumor tissue samples from all groups compared to control, and in MIBC compared to NMIBC, with significant relations between their levels and LN involvement. Caspase-3 was significantly downregulated in HGBC compared to LGBC. Levels of ATG5, CHOP and LC3A can discriminate between normal and malignant urothelial tissue and between invasive and non-invasive bladder cancer.

conclusionExpression of tissue autophagy biomarkers is associated with aggressive clinicopathological features (muscle invasion) in bladder cancer.

Indexed as

Autophagy-Related Protein 5Microtubule-Associated ProteinsTranscription Factor CHOPUrinary Bladder NeoplasmsAgedAutophagyBiomarkers, TumorCaspase 3FemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedNeoplasm InvasivenessNon-Muscle Invasive Bladder NeoplasmsATG5 protein, humanAutophagy-Related Protein 5Biomarkers, TumorCaspase 3DDIT3 protein, humanMAP1LC3A protein, humanMicrotubule-Associated ProteinsTranscription Factor CHOPAutophagyBiomarkersCancerMalignant neoplasmMuscle invasion

Identifiers

PMID42507049

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.