Evidence map›Paper›PMID 42507160›Full record

ArticleWorld journal of urology2026

Piezo1 promote macrophage-to-myofibroblast transition and bladder fibrosis in neurogenic bladder.

Shaoguang Feng, Yehang Zhu, Huajun Yang, Dongsheng Zhu, Congde Chen

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Article in World journal of urology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shaoguang FengDepartment of Pediatric Surgery, Hangzhou Children's Hospital, 195 Wenhui Road, Hangzhou, 310014, Zhejiang, China.
Yehang ZhuDepartment of Pediatric Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Huajun YangDepartment of Pediatric Surgery, Hangzhou Children's Hospital, 195 Wenhui Road, Hangzhou, 310014, Zhejiang, China.
Dongsheng ZhuDepartment of Pediatric Surgery, The First People's Hospital of Lianyungang, Affiliated to Kangda College of Nanjing Medical University, Lianyungang, 222000, Jiangsu, China. zhudongsheng@tmu.edu.cn.
Congde ChenDepartment of Pediatric Urology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Funding

Department of Health of Zhejiang Province 2025HY0755Hangzhou Key Discipline (Peak Discipline - Fetal and Neonatal Medicine) 2025HZGF03
6 · The paper itself

Abstract

backgroundNeurogenic bladder (NB) frequently leads to bladder fibrosis, yet the underlying mechanisms remain unclear. Piezo1, a mechanosensitive ion channel, has been implicated in fibrotic processes. This study investigated whether Piezo1 promotes macrophage-to-myofibroblast transition (MMT) and contributes to bladder fibrosis in NB.

methodsA mouse NB model was established via spinal cord injury. Bladder tissues were analyzed by HE and Masson staining, immunohistochemistry, qRT-PCR, and Western blot to assess fibrosis and Piezo1 expression. Immunofluorescence colocalization and flow cytometry were used to evaluate MMT. In vitro, RAW264.7 macrophages were treated with TGF-β1, the Piezo1 agonist Yoda1, the Piezo1 inhibitor GsMTx4, or Piezo1 siRNA, followed by assessment of MMT and fibrosis-related protein expression.

resultsPiezo1 expression was significantly upregulated in NB bladder tissues, correlating with increased collagen deposition and fibronectin expression. Immunofluorescence revealed colocalization of macrophage markers (CD68, F4/80) with α-SMA, indicating MMT in NB tissues. In vitro, TGF-β1-induced MMT was enhanced by Yoda1 and attenuated by GsMTx4 or Piezo1 knockdown, as confirmed by immunofluorescence and flow cytometry. Similarly, Piezo1 activation increased the expression of fibrosis-related proteins (Fibronectin, Collagen I, α-SMA), whereas Piezo1 inhibition suppressed their expression.

conclusionsPiezo1 is a critical regulator of macrophage-to-myofibroblast transition and promotes bladder fibrosis in neurogenic bladder. Targeting Piezo1 may represent a potential therapeutic strategy to mitigate fibrosis and preserve bladder function in NB patients.

Indexed as

Ion ChannelsMacrophagesMyofibroblastsUrinary BladderUrinary Bladder, NeurogenicAnimalsDisease Models, AnimalFemaleFibrosisMiceMice, Inbred C57BLIon ChannelsPiezo1 protein, mouseFibrosisMacrophage-to-myofibroblast transition (MMT)Neurogenic bladderPiezo1

Identifiers

PMID42507160

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.