Evidence mapPaperPMID 42507693Full record

ArticlePloS one2026

Bacterial profile and antimicrobial resistance patterns of bloodstream infections at Amhara Public Health Institute, Bahir Dar, Ethiopia.

Michael Getie, Wudu Tafere, Alem Tsega, Tsehaynesh Gebreyesus, Gizeaddis Belay, Hailu Getachew, Yosef Gashaw, Aimro Tadese, Tigist Birku, Tigabu Birhan and 4 more

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Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

14 authors.

Michael GetieMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.ORCID https://orcid.org/0000-0001-7461-859X
Wudu TafereMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Alem TsegaMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Tsehaynesh GebreyesusMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Gizeaddis BelayMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Hailu GetachewMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Yosef GashawMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.ORCID https://orcid.org/0009-0002-4553-7955
Aimro TadeseMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Tigist BirkuMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Tigabu BirhanMedical Microbiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Alemayehu AbateMedical Biotechnology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Teshiwal DeressDepartment of Quality Assurance and Laboratory Management, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Gebremedhin NechoField Epidemiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.
Belay BezabihField Epidemiology, Amhara Public Health Institute, Bahir Dar, Ethiopia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundManaging bloodstream infections in resource-constrained regions like Ethiopia is challenging due to scarce blood culture surveillance data. To guide empirical therapy, this study determines the bacterial profiles and antimicrobial resistance patterns among patients with suspected bloodstream infections at the Amhara Public Health Institute.

methodsThis retrospective study analyzed blood culture records from the Amhara Public Health Institute spanning January 1, 2020, to December 30, 2023. Blood samples were processed using standardized manual culture techniques in accordance with World Health Organization (WHO) protocols. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analysis was conducted using SPSS version 20, employing descriptive statistics and regression models, with statistical significance defined as p < 0.05.

resultsTrue bacterial pathogens were isolated from 50.3% (N = 340) of the 676 patients, with Gram-negative bacteria predominating (61.2%, N = 208) over Gram-positive bacteria (38.8%, N = 132). Notably, ESKAPEE pathogens accounted for 96.5% (N = 328) of all isolates, led by Klebsiella spp. (25.9%, N = 88), Enterococcus spp. (20.9%, N = 71), and S. aureus (15.6%, N = 53). Among Gram-positive isolates, high resistance was observed against oxacillin (74.5%, N = 41), penicillin (72%, N = 36), and vancomycin (51.1%, N = 24). Gram-negative isolates exhibited critical resistance to national frontline therapeutics, including ampicillin (100%, N = 24), ceftriaxone (92.4%, N = 157), and trimethoprim-sulfamethoxazole (89.4%, N = 126). Overall, multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR) profiles were detected in 43.8% (N = 149), 30.5% (N = 104) and 5.3% (N = 18) of isolates with similar distributions observed among ESKAPEE strains. Sex and age were the only independent predictors of culture positivity, with female sex reducing bloodstream infection odds by 44% (p = 0.002). Conversely, neonates (≤ 28 days) and young adults (15-24 years) had 4.7 times (p = 0.001) and 9.5-times (p = 0.001) higher odds compared to elderly patients.

conclusionsThis study reveals a severe 50.3% (N = 340) bloodstream infection rate dominated by highly resistant Gram-negative and ESKAPEE pathogens, disproportionately affecting males, neonates, and young adults. Combating this threat requires immediate antibiotic stewardship, updated guidelines, and advanced laboratory testing (anaerobic culture, minimum inhibitory concentration, and molecular sequencing) to track and manage resistance.

Indexed as

Anti-Bacterial AgentsBacteremiaDrug Resistance, BacterialAdolescentAdultAgedChildChild, PreschoolEthiopiaFemaleGram-Negative BacteriaGram-Positive BacteriaHumansInfantMaleMicrobial Sensitivity TestsAnti-Bacterial AgentsThird Generation Cephalosporins

Identifiers

PMID42507693
PMCPMC13405292

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.