ArticleNature methods2026
Spike inference from calcium imaging data acquired with GCaMP8 indicators.
Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Functional and structural characterization of dendritic spine pathology in a mouse model of tauopathy.iScience · 2026Article
- A cortical output channel for perceptual categorization.Science advances · 2026Article
- Distinct types of dendritic CaScience advances · 2026Article
- Intrinsic Diversity in Odor-Evoked Calcium Rises acrossThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Improving positively tuned voltage indicators for faster kinetics and higher contrast.bioRxiv : the preprint server for biology · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Calcium imaging is a central method in neuroscience but it records neuronal activity only indirectly and thereby produces results that are difficult to interpret. Here we evaluate the GCaMP8 calcium indicator variants, together with methods to infer neuronal spiking, and thus interpret GCaMP8 recordings. We find that the linearity of GCaMP8 indicators enables accurate detection of both single action potentials and high-frequency spiking events. Ground-truth recordings from mouse neocortex show that the most linear variants GCaMP8s and GCaMP8m (but not GCaMP6, GCaMP7f or GCaMP8f) robustly detect isolated spikes in cortical pyramidal neurons. In addition, we fine-tune and benchmark algorithms for spike inference (CASCADE, OASIS and MLSpike) with data from all GCaMP8 variants for pyramidal neurons and interneurons, and we demonstrate how the fast rise time of GCaMP8 indicators enables real-time detection of neuronal activity. Overall, we provide tools and guidelines to optimally process GCaMP8 calcium signals and highlight the key role of linearity in interpreting calcium imaging data.
Identifiers
42509475What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.