A Multi-Task Deep Learning Framework for Characterizing Beating Behavior and Synchrony in Cardiomyocyte Clusters.

Beat-level synchrony among cardiomyocyte clusters is a critical indicator of cardiac electromechanical function. Traditional invasive approaches have substantial limitations, and conventional computer vision methods are poorly suited for resolving densely packed, adherent clusters. To address these challenges, we developed an analysis framework to characterize the beating characteristics of cardiomyocyte clusters from microscopic imaging data. Specifically, we propose CardioSegNet, a multi-task deep learning model that combines attention mechanisms with three prediction heads (semantic segmentation, contour detection, and distance transform), followed by a watershed algorithm to achieve high-accuracy cluster-level segmentation of cardiomyocyte clusters. The Pixel-Difference method is applied to extract time-series beating signals from each segmented cluster and compute several dynamic parameters, including beating amplitude, period, frequency, and the Beat Rate Irregularity (BRI). We further introduce PeriodAwareNAPTD