Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.

Over a decade of research on media for cultured meat and seafood production has resulted in multiple highly efficient serum-free and chemically defined formulations for some species, but it has also identified challenges yet to be solved-especially for aquatic cell lines. Depending on the product and cell type, the approach to develop highly efficient, sustainable, and low-priced media can diverge greatly. In this review, we provide an in-depth overview of this complex research area to facilitate strategic decision-making for stakeholders. We evaluate the advantages and limitations of utilizing hydrolysates, growth factor mutants, growth factor alternatives, and stabilizers in serum-free media formulations published for cultured meat production, as well as ongoing research efforts on developing adequate media for cultured seafood. We critically analyze strategies aimed at reducing medium costs and enhancing sustainability of cultured meat and seafood production, including their food-compatibility assessment. We summarize topics that require further exploration, such as identification of species-specific growth factors-particularly for aquatic species; exploration of hydrolysates as a substitute for basal medium; waste medium recycling strategies; and the potential application of artificial intelligence (AI) and machine learning (ML) technologies to enhance these areas. Additionally, we consider possible emerging regulatory issues and their impact on media formulation development. Finally, key performance indicators for media formulations are proposed to guide future strategic and operational improvements regarding an economical and sustainable production process.