ArticleTranslational animal science2026
Multi-protease supplementation enhances growth performance and promotes gut health in broilers fed low-protein, low-amino acid diets.
Article in Translational animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The objective of this study was to assess the impact of dietary supplementation with a multi-protease enzyme on broiler diets with reduced levels of protein and amino acids. One-day-old male Cobb-500 chicks (2250 birds) were assigned to 10 treatments (9 pens/treatment). The treatments included (1) a nutritionally adequate Positive Control (PC); (2) PC + 200 g/t of a multi-protease (KZP); (3) a Negative Control (NC1) reformulated to-4% PC and amino acids; (4) NC1 + 100 g/t KZP; (5). NC1 + 200 g/t KZP; (6). NC1 + 300 g/t KZP; (7) a Negative Control (NC2) reformulated to - 8% PC and amino acids; (8). NC2 + 100 g/t KZP; (9) NC2 + 200 g/t KZP; and (10) NC2 + 300 g/t KZP. NC1 and NC2 showed the worst growth performance, with supplementation of KZP able to restore performance to the level of the PC at 200 g/t and 300 g/t dosage. There was no effect on carcass yield. The inclusion of multi-protease enhanced intestinal morphology, with increased villus height and villus: crypt ratio, along with reduced crypt depth. Protease supplementation promoted descriptive taxonomic shifts in cecal microbiota composition. Multi-protease enhanced bird performance, gut morphology, and influenced microbial community composition in amino acid-reduced diets.
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