ArticlePhotoacoustics2026
Quantitative assessment of gastrointestinal motility in neonatal rats by real-time 3D photoacoustic imaging: A feasibility study.
Article in Photoacoustics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Gastrointestinal (GI) motility assessment in neonates remains challenging for conventional clinical imaging. We present a preliminary quantitative framework for GI dynamics in healthy neonatal rats using a real-time three-dimensional photoacoustic imaging system, enabling high-contrast visualization of gastric and intestinal structures. India ink was administered orally as a contrast agent to enhance visualization of the GI tract. We developed a dual-branch analysis pipeline including spatiotemporal kymography for local rhythmic activity quantification and cluster tracking for transport-pattern classification. In healthy neonatal rats, intestinal rhythmic activity was measured at 9.25 ± 0.96 cycles per minute, while gastric peristaltic waves were identified from kymographs. Tracking of contrast-agent clusters revealed local propulsive and retrograde transport patterns, while segmental-like activity was identified from cluster-splitting events. This feasibility study demonstrates a volumetric GI imaging and analysis method for neonatal GI kinematics, laying a foundation for future larger-scale validation and disease-model studies.
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