Production of gene-edited cloned cattle embryos using the CRISPR/EOCas12i system.

Furui Wang, Lei Chen, Yuting Ning, Jiale He, Yinjuan Wang, Lei An, Jianhui Tian, Guangyin Xi

Abstract read

In one paragraph

Article in Frontiers in genome editing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Furui Wang *College of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Lei Chen *College of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Yuting NingCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Jiale HeCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Yinjuan WangCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Lei AnCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Jianhui TianCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Guangyin XiCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The rapid development of genome editing technologies has enabled precise manipulation of livestock genomes for the improvement of production traits such as meat yield and milk quality. Myostatin ( Methods: In this study, we employed an optimized CRISPR/EOCas12i system to simultaneously target Results: T7E1 and Sanger sequencing confirmed efficient editing at multiple target sites, with EOCas12i producing deletions ranging from tens to over 100 bp. Furthermore, no off-target (OT) effects were detected at predicted loci, supporting the high specificity of this system in large animals. Gene-edited single-cell clones (SCCs) were expanded in conditioned medium, and selected double-knockout (DKO) clones served as nuclear donors for somatic cell nuclear transfer (SCNT) to produce Discussion: Collectively, this study demonstrates the feasibility of generating

Indexed as

BLG genecattleCRISPR/EOCas12iMSTN geneSCNT

Identifiers

PMID42519492

DOI10.3389/fgeed.2026.1891100

PMCPMC13381622

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.