Evidence map›Paper›PMID 42519544›Full record

ArticleCell press blue2026

A spatial multi-omics atlas of immunosenescence reveals germinal-center B cell dysfunction in human lymph nodes.

Negin Farzad, Archibald Enninful, Yao Lu, Fabio Parisi, Anthony Fung, Yumi Kwon, Yajuan Li, Margaux Labrosse, Mingyu Yang, Francesco Strino and 23 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell press blue, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

33 authors.

Negin FarzadDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Archibald EnninfulDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Yao LuDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Fabio ParisiComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Anthony FungDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Yumi KwonEnvironmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Yajuan LiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
Margaux LabrosseRESTORE, Research Center, Team 1 STROMAGICS, Université de Toulouse, INSERM, CNRS, EFS, ENVT, Université P. Sabatier, Toulouse, France.
Mingyu YangDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Francesco StrinoComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Liang ChenEnvironmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Junchen YangComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Mei ZhongDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Fu GaoDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Bo TaoDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Joseph CunninghamComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Zhiliang BaiDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Haikuo LiDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Fang WangDepartment of Internal Medicine, Yale School of Medicine, New Haven, CT 06520, USA.
Michael StankewichDepartment of Pathology, Yale School of Medicine, New Haven, CT 06520, USA.
Dongjoo KimDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
Mingze DongComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Lisa M BramerBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Keyi LiComputational Biology and Bioinformatics Program, Yale University, New Haven, CT 06520, USA.
Meera R BhatDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Evan LoeDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Joseph CraftDepartment of Internal Medicine, Yale School of Medicine, New Haven, CT 06520, USA.
Ljiljana Pasa-TolicEnvironmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Stephanie HaleneYale Center for Research on Aging Biology, Yale School of Medicine, New Haven, CT 06520, USA.
Lingyan ShiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
Yuval KlugerYale Center for Research on Aging Biology, Yale School of Medicine, New Haven, CT 06520, USA.
Mina L XuDepartment of Pathology, Yale School of Medicine, New Haven, CT 06520, USA.
Rong FanDepartment of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.

Funding

Spatiotemporal Tumor Analytics for Guiding Sequential Targeted-Inhibitor: Immunotherapy Combinations (ST-Analytics)U54CA274509 · NCI · INSTITUTE FOR SYSTEMS BIOLOGY · PI Rong Fan · 2022 to 2026
$15.6M
Yale TMC for Cellular Senescence in Lymphoid OrgansU54AG076043 · NIA · YALE UNIVERSITY · PI FAN, RONG, HALENE, STEPHANIE · 2021 to 2025
$7.0M
Yale Murine-TMC on Immune Cell Senescence Derived InflammationU54AG079759 · NIA · YALE UNIVERSITY · PI DIXIT, VISHWA DEEP, MONTGOMERY, RUTH R · 2022 to 2025
$6.5M
High-throughput in vivo and in vitro functional and multi-omics screens of neuropsychiatric and neurodevelopmental disorder risk genesRM1MH132648 · NIMH · YALE UNIVERSITY · PI Kristen Jennifer Brennand, Rong Fan · 2023 to 2026
$5.6M
Center for Human Lymphoma Spatiotemporal Atlas (HuLymSTA)U01CA294514 · NCI · YALE UNIVERSITY · PI FAN, RONG, HALENE, STEPHANIE · 2024 to 2025
$5.1M
Highly scalable and sensitive spatial transcriptomic and epigenomic sequencing of brain tissues from human and non-human primateRF1MH128876 · NIMH · YALE UNIVERSITY · PI FAN, RONG, SESTAN, NENAD · 2021 to 2021
$2.9M
Ex vivo analysis of human brain tumor cells in a microvascular niche modelR01CA245313 · NCI · YALE UNIVERSITY · PI FAN, RONG, ZHOU, JIANGBING · 2020 to 2024
$2.6M
High-spatial-resolution ECM-inclusive multi-omics sequencing of human PFA and FFPE tissue slidesUH3CA257393 · NCI · YALE UNIVERSITY · PI FAN, RONG · 2022 to 2023
$1.2M
NCI NIH HHS R01 CA245313NCI NIH HHS U01 CA294514NCI NIH HHS U54 CA274509NCI NIH HHS UH3 CA257393NIA NIH HHS U54 AG076043NIA NIH HHS U54 AG079759NIMH NIH HHS RF1 MH128876NIMH NIH HHS RM1 MH132648
6 · The paper itself

Abstract

Immunosenescence is a hallmark of human aging and contributes to age-related immune decline, yet the development of senescence-associated phenotypes in the human lymphoid organs remains poorly understood. Here, we integrate single-cell and spatial multi-omics to systematically characterize age-related senescence in human lymph nodes (LNs) across the lifespan. Spatial proteomic profiling of 99 LN sections from 51 donors (18-86 years) using high-plex immunofluorescence (~20 million cells) mapped senescence markers (p16, p21, HMGB1, and

Identifiers

PMID42519544
PMCPMC13384480

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.