ArticleFrontiers in plant science2026
The efficient plant genetic transformation system mediated by FELBs and RTBs respectively
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Somatic embryogenesis represents an indirect but highly efficient biotechnological method for plant regeneration, genetic transformation, and virus-free propagation. Methods: Herein, we investigated the effects of NAA, 2,4-D, and TDZ on the morphogenic capacity of Results: Two novel high-efficiency SE systems in garlic were characterized, operating through the FELB and RTB pathways, respectively, with a focus on the regulatory effects of plant growth regulators (PGRs) on SE progression. In the FELB pathway, somatic embryos were highly induced from root explants, achieving an induction rate of 96.33%. Embryoid development was successfully accomplished using a combination of 2.5 mg/L NAA and 2.5 mg/L 2,4-D under dark conditions. Furthermore, 5.0 mg/L BAP was identified as the optimal cytokinin concentration for plant regeneration Discussion: Collectively, our findings establish novel methodologies for garlic SE and transgenic manipulation, providing valuable tools for the genetic improvement of this economically important crop.
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