ArticleSTAR protocols2026
Protocol for CRISPR genome editing in S. cerevisiae using PCR-based guide insertion.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
6 authors.
Funding
Abstract
Here, we present a CRISPR-Cas9 genome-editing protocol for Saccharomyces cerevisiae that simplifies guide construction and enables rapid guide iteration by replacing restriction/ligation cloning with PCR-based guide installation and seamless Cas9-plasmid recircularization. We describe single-guide RNA (sgRNA) and homology-directed repair (HDR) donor design, guide insertion into a KanMX/G418-selectable Cas9-sgRNA plasmid, plasmid cloning and sequence verification in E. coli, and lithium acetate/polyethylene glycol (LiAc/PEG) co-transformation of yeast with the verified plasmid and HDR donor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.