Evidence map›Paper›PMID 42529578›Full record

ArticleChemical & biomedical imaging2026

Impact of Histological Processing on Endogenous Elemental Contents and Distributions in Breast Cancer Tissue Sections.

Amy V Lovejoy, Alexander P Morrell, Diego Mesa, Alex Griffiths, Piotr R Golda, Kay L Dawson, Evie Wren, Katharina Kreissig, Charles Coombes, Mark Rehkämper and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Bioimaging of Metals.Chemical & biomedical imaging · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Amy V LovejoyDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0000-0003-4079-942X
Alexander P MorrellLondon Metallomics Facility, King's College London, London SE1 9NH, U.K.ORCID https://orcid.org/0000-0003-3763-5846
Diego MesaDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0000-0002-2417-9821
Alex GriffithsDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0000-0002-6772-2054
Piotr R GoldaLondon Metallomics Facility, King's College London, London SE1 9NH, U.K.ORCID https://orcid.org/0009-0002-1380-9047
Kay L DawsonImperial College Healthcare Tissue Bank, Charing Cross Hospital Campus, London W6 8RP, U.K.
Evie WrenImperial College Healthcare Tissue Bank, Charing Cross Hospital Campus, London W6 8RP, U.K.ORCID https://orcid.org/0009-0008-3361-0274
Katharina KreissigDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0009-0000-8913-7924
Charles CoombesDepartment of Surgery and Cancer, Hammersmith Hospital, Imperial College London, London W12 0NN, U.K.ORCID https://orcid.org/0000-0002-4811-1100
Mark RehkämperDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0000-0002-0075-9872
Rebekah E T MooreDepartment of Earth Science and Engineering, Imperial College London, London SW7 2AZ, U.K.ORCID https://orcid.org/0000-0002-9056-8980

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trace metal homeostasis is critical for cellular function and often disrupted in diseases such as cancer. Spatially resolved techniques enable mapping of metal distributions within complex cellular environments, surpassing the limitations of bulk analyses. Formalin-fixed, paraffin-embedded (FFPE) tissues are widely used in research, yet the impact of FFPE processing on endogenous elemental contents and distributions remains unclear, with conflicting findings across sample types. This study evaluates the suitability of FFPE human breast carcinoma tissue sections for multielemental bioimaging by direct comparison with paired fresh frozen (FF) tissues and assesses potential sources of contamination or elemental losses during processing. Three FF human breast carcinoma specimens were bisected and processed as either FF or FFPE. Serial sections underwent analyses by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), alongside hematoxylin and eosin (H&E)-staining, to compare elemental profiles in whole sections and morphologically distinct regions. In parallel, FFPE processing reagents encompassing tissue-marking dyes, formalin, paraffin wax, xylene, and ethanol were analyzed via solution ICP-MS to contextualize the

Indexed as

breast cancerelemental imagingformalin-fixed paraffin embedding (FFPE)laser ablation ICP-MStissue processing

Identifiers

PMID42529578
PMCPMC13417537

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.