Evidence map›Paper›PMID 42530754›Full record

ArticleTropical animal health and production2026

Non-genetic determinants and interaction effects on semen quality and production efficiency in Mehsana buffalo bulls: a large-scale field study.

N S Dangar, B P Brahmkshtri, P A Patel, H A Modh

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Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

N S DangarCollege of Veterinary Science and A. H, Kamdhenu University, Bhuj, Gujarat, 370001, India. drnik2487@gmail.com.ORCID http://orcid.org/0000-0001-8185-7361
B P BrahmkshtriCollege of Veterinary Science and A. H, Kamdhenu University, Himmatnagar, Gujarat, India.
P A PatelPashu Samvardhan Kendra, Dudhsagar Dairy, Mehsana, Gujarat, 384001, India.
H A ModhDama Semen Production Unit, Banas Dairy, Dama, Gujarat, 385535, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding the environmental and management-related determinants of semen production is essential for optimizing artificial insemination programs in buffalo breeding. This study comprehensively evaluated the influence of major non-genetic factors and their interactions on semen quality and semen production traits in Mehsana buffalo bulls using one of the largest repeated-record field datasets reported for this breed. A total of 55,071 ejaculates collected from 244 bulls maintained at two organized semen stations in Gujarat, India, were analyzed. Semen volume, sperm concentration, initial sperm motility, post-thaw sperm motility, and the number of semen doses produced per ejaculate were evaluated using repeated-record linear mixed-effects models fitted by restricted maximum likelihood (REML), with bull included as a random effect and within-bull correlations modeled using a first-order autoregressive covariance structure. Age at semen collection, period of semen collection, and ejaculate number consistently influenced all semen traits, whereas farm and season exhibited trait-specific effects. Semen volume and semen dose yield generally increased with advancing age, peaked between 3 and 5 years (197-202 semen doses per ejaculate), and declined thereafter. The first ejaculate consistently produced greater semen volume, sperm concentration, and semen dose output than the second ejaculate (248 vs. 115 semen doses per ejaculate). Significant interactions among farm, age, season, and ejaculate number demonstrated that semen traits responded differently across management environments and physiological stages, supporting age-specific bull utilization and optimized semen collection schedules in organized breeding programmes. By integrating a large longitudinal dataset with repeated-record mixed-model analyses, this study provides a comprehensive characterization of non-genetic sources of variation affecting semen production traits in Mehsana buffalo bulls. The findings offer an evidence-based framework for optimizing bull utilization and semen collection strategies in organized breeding programs while highlighting the need for future investigations integrating fertility, physiological, environmental, and genomic information to elucidate the mechanisms underlying the observed associations.

Indexed as

BuffaloesSemenSemen AnalysisAnimalsBreedingIndiaInsemination, ArtificialMaleSeasonsSperm CountSperm MotilityArtificial insemination efficiencyBubalus bubalisMehsana breedNon-genetic variationSemen quality parametersSperm cryosurvival

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.