Evidence map›Paper›PMID 42537649›Full record

ArticleCell reports methods2026

Targeting the peripheral nervous system in adipose tissue with engineered AAV vectors.

Jake W Willows, Lindsey M Lazor, Gilian Gunsch, Gargi Mishra, Andrew P Villa, Timothy F Shay, Andrew D Steele, Kristy L Townsend

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jake W WillowsDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, USA.
Lindsey M LazorDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, USA.
Gilian GunschDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, USA.
Gargi MishraDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, USA.
Andrew P VillaBiological Sciences Department, California State Polytechnic University Pomona, Pomona, CA, USA.
Timothy F ShayDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Andrew D SteeleBiological Sciences Department, California State Polytechnic University Pomona, Pomona, CA, USA.
Kristy L TownsendDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, USA. Electronic address: kristy.townsend@osumc.edu.

Funding

Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences - SUPPLEMENTU24MH131054 · NIMH · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GRADINARU, VIVIANA, SHAY, TIMOTHY FRANCIS · 2022 to 2024
$1.9M
NIMH NIH HHS U24 MH131054
6 · The paper itself

Abstract

Adipose tissue innervation plays a critical role in regulating energy homeostasis and metabolic functions, yet targeted gene delivery to these peripheral nerves has remained challenging. We systematically evaluated both naturally occurring and engineered adeno-associated virus (AAV) capsids for their ability to transduce nerve fibers in the adipose tissue of mice. We compared seven AAVs (AAV1, AAVrg, AAV5, AAV9, AAV-PHP.S, AAV-MaCPNS1, and AAV-MaCPNS2) in C57BL/6J mice and identified AAV-PHP.S as highly efficient for transducing nerves within inguinal subcutaneous white adipose tissue (ing-scWAT). Titration studies further optimized intra-adipose delivery to minimize off-target expression while selectively targeting adipose tissue nerves. Building on this optimization, we employed Cre-dependent AAVs to selectively target Nav1.8

Indexed as

Adipose TissueDependovirusGenetic VectorsPeripheral Nervous SystemAnimalsGene Transfer TechniquesMiceMice, Inbred C57BLTransduction, GeneticAAVadeno-associated virusadiposecapsidCP: neuroscienceDREADDgene therapyperipheral nervous systemserotype

Identifiers

PMID42537649
PMCPMC13615495

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.