ArticlemicroPublication biology2026
Improved chromatographic separation of modified deoxycytidine nucleosides enhances LC-MS sensitivity.
Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Cytosine methylation is an epigenetic modification that regulates transcription. DNA demethylation occurs through oxidation of methylcytosine to hydroxymethylcytosine, making hydroxymethylation an important and translationally relevant marker of DNA methylation dynamics. These epigenetic modifications can be measured in their deoxynucleoside forms from enzymatically digested DNA by liquid chromatography-mass spectrometry (LC-MS). However, 5-hydroxymethyl-2'-deoxycytidine (5hmdC) co-elutes with 2'-deoxycytidine (dC). Because 5hmdC represents <0.05% of total dC in DNA, co-elution with dC causes ion suppression that limits detection and quantitation. Here, we present a chromatographic method that separates 5hmdC from dC, eliminates ion suppression, and improves the sensitivity of 5hmdC detection.
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.