Evidence mapPaperPMID 42540656Full record

ArticleDrug design, development and therapy2026

Probiotics in Combination with C16 Peptide and Angiopoietin-1 Synergistically Ameliorate Multiple Sclerosis in Mice.

Qiang Li, Yi Zhao, Lu Bai, Lu-Ping Tang, Shu Han

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Article in Drug design, development and therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Qiang LiThe Emergency Department, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, People's Republic of China.
Yi ZhaoThe Emergency Department, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, People's Republic of China.
Lu BaiInstitute of Anatomy, Medical College, Zhejiang University, Hangzhou, Zhejiang, 310058, People's Republic of China.
Lu-Ping TangThe Emergency Department, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, People's Republic of China.ORCID 0000-0003-4255-9921
Shu HanInstitute of Anatomy, Medical College, Zhejiang University, Hangzhou, Zhejiang, 310058, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Multiple sclerosis (MS) is a debilitating autoimmune disease of the central nervous system (CNS), characterized by demyelination, axonal loss, and neuronal injury. At present, effective treatment options remain limited. Prior research demonstrated the protective effects of a compound that consists of C16 peptide and angiopoietin-1 (C16-Ang-1) in experimental autoimmune encephalomyelitis (EAE), a validated animal model of MS. This study aims to investigate the potential synergistic effects of probiotics with C16+Ang-1, and elucidate its underlying mechanisms in mice. Methods: C57/BL6 mice were randomly assigned to control, vehicle, probiotics, and C16+Ang-1+probiotics groups. Histological examinations, behavioral tests, and 16S rRNA gene sequencing of fecal samples, were conducted to determine the levels of CNS inflammation, demyelination and axonal loss, neuronal survival, and functional recovery. Results: Compared to the probiotics group, the C16+Ang-1+probiotics group exhibited significant synergistic effects. Specifically, the combined treatment with C16+Ang-1+probiotics had significantly greater effects than probiotics alone in reducing inflammatory severity in the CNS and colon, improving the microenvironment, protecting the gut-blood barrier, and preserving blood-brain barrier integrity. These effects collectively led to the greater amelioration of functional disability in the mouse model of MS. Further mechanistic studies suggested that these effects involved the modulation of the brain-gut axis, and maintenance of gut microbiota homeostasis. Conclusion: These findings demonstrate that the combination of probiotics and C16-Ang-1 acts synergistically to ameliorate MS in mice, resulting in a greater effect. Therefore, this combination therapy warrants further investigation to explore its potential clinical benefits, ultimately improving the care for patients with MS.

Indexed as

Angiopoietin-1Encephalomyelitis, Autoimmune, ExperimentalMultiple SclerosisPeptide FragmentsPeptidesProbioticsAnimalsDisease Models, AnimalFemaleMiceMice, Inbred C57BLAngiopoietin-1Peptide FragmentsPeptidesangiopoietin-1C16 peptideexperimental autoimmune encephalomyelitisgut-brain axisinflammationmultiple sclerosisprobiotic

Identifiers

PMID42540656
PMCPMC13426507

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.