ArticleAntonie van Leeuwenhoek2026
Endophytic fungus Muyocopron dipterocarpi isolated from Catharanthus roseus as a novel producer of ajmalicine and other metabolites: insights from HPLC and LC-HRMS analysis.
Article in Antonie van Leeuwenhoek, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Endophytic fungi are mutualistic microorganisms that live within plant tissues without harming the host. They enhance plant health and are valuable sources of bioactive secondary metabolites for medicinal use. This study aimed to identify the endophytic fungi in the medicinal plant Catharanthus roseus, collected from different locations in Jharkhand, India. A total of thirty unique fungal isolates were obtained and maintained through continuous subculturing. Among them, Muyocopron dipterocarpi has been selected for further chemical profiling based on its promising bioactive potential. Primary screening of the metabolite was done through alkaloid test and HPTLC analysis. A comprehensive metabolite profiling of the selected isolate has been performed by employing advanced analytical techniques such as high-performance liquid chromatography, gas chromatography-tandem mass spectrometry, and liquid chromatography-high resolution mass spectrometry. The GC-MS/MS analyses of extracts from each species identified multiple bioactive compounds, including 1-tetradecene, 2,4-Di-tert-butylphenol, 1-hexadecanol, ethyl iso-allocholate, digitoxin, and 1-hexacosene. Using LC-HRMS for comprehensive profiling of fungal metabolites, we identified several indole alkaloids from Muyocopron dipterocarpi for the first time, including gelsemicine, ajmaline, brassitin, and geissospermine. Among the metabolites detected, ajmalicine was found to be the predominant monoterpenoid indole alkaloid present in the crude extract. Structural confirmation was established via Orbitrap high-resolution mass spectrometry, which provided evidence of a unique sodium adduct ion at m/z 375.1686, which matches the theoretical mass. Additionally, HPLC quantification of ajmalicine indicates that it is the primary indole alkaloid in the selected groups, with an estimated concentration of 106.1 µg/mL.
Indexed as
Identifiers
42545508What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.