Evidence map›Paper›PMID 42548660›Full record

ArticleFrontiers in immunology2026

Fibrotic remodeling in the NOD/ShiLtJ mouse model of Sjögren's disease: insights from single-cell transcriptomics and AI-driven ECM quantification.

Jennifer M Morrissey, Deirdre A Nelson, Li Chen, Mathieu Petitjean, Joey R Tavarez, Amber L Altrieth-Flagg, Nicholas L Moskwa, Renae Williams-Atkinson, Ben Fowler, Rafael Pena and 4 more

Abstract read
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jennifer M MorrisseyMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.
Deirdre A NelsonDepartment of Biological Sciences and The RNA Institute, State University of New York, University at Albany, Albany, NY, United States.
Li ChenPharmaNest Inc., Princeton NJ, United States.
Mathieu PetitjeanPharmaNest Inc., Princeton NJ, United States.
Joey R TavarezMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.
Amber L Altrieth-FlaggMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.
Nicholas L MoskwaDepartment of Biological Sciences and The RNA Institute, State University of New York, University at Albany, Albany, NY, United States.
Renae Williams-AtkinsonDepartment of Biological Sciences and The RNA Institute, State University of New York, University at Albany, Albany, NY, United States.
Ben FowlerImaging Facility and Histology Core, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States.
Rafael PenaMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.
Kennedi WestonMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.
Nikhita KumarDepartment of Biological Sciences and The RNA Institute, State University of New York, University at Albany, Albany, NY, United States.
Nathan AistPharmaNest Inc., Princeton NJ, United States.
Melinda LarsenMolecular, Cellular, Developmental, and Neural Biology Graduate Program, State University of New York, University at Albany, Albany, NY, United States.

Funding

Remediating fibrosis for salivary gland regenerationR01DE027953 · NIDCR · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI MELINDA LARSEN · 2019 to 2026
$5.4M
Cellular plasticity in salivary gland regeneration.R01DE030626 · NIDCR · UNIVERSITY OF ROCHESTER · PI LARSEN, MELINDA · 2021 to 2025
$2.3M
Evident SLIDEVIEW VS200 Slide ScannerS10OD036207 · OD · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI SCIMEMI, ANNALISA · 2025 to 2025
$278k
NIDCR NIH HHS R01 DE027953NIDCR NIH HHS R01 DE030626NIH HHS S10 OD036207
6 · The paper itself

Abstract

Introduction: Sjögren's Disease (SjD) is an autoimmune disorder characterized by salivary gland hypofunction and lymphocytic infiltration, yet the contribution of fibrosis to glandular dysfunction remains unclear. Methods: We evaluated the NOD/ShiLtJ mouse as a model for salivary gland fibrosis and used it to examine the impact of antifibrotic therapy on extracellular matrix (ECM) remodeling. To assess therapeutic potential, we treated NOD/ShiLtJ female mice with nintedanib, an FDA-approved antifibrotic agent used to treat chronic fibrosing interstitial lung disease. Fibrosis was assessed using single-cell RNA sequencing combined with Picrosirius red staining and AI-assisted digital pathology quantification of collagen I patterning with FibroNest. Results: Single-cell RNA sequencing revealed that fibroblast populations in the submandibular and sublingual salivary glands of the female NOD/ShiLtJ mouse exhibited increased levels of ECM genes, including Discussion: Collectively, these findings establish the NOD/ShiLtJ strain as a model of SjD-associated salivary gland fibrosis and provide proof-of-concept evidence supporting antifibrotic strategies as a potential therapeutic avenue for salivary gland dysfunction in SjD.

Indexed as

Extracellular MatrixSalivary GlandsSjogren's SyndromeTranscriptomeAnimalsDisease Models, AnimalFemaleFibrosisIndolesMiceMice, Inbred NODSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisIndolesnintedanibextracellular matrixfibrosisnintedanibNOD/ShiLtJ mousesalivary glandsingle-cell RNA sequencingSjögren’s disease

Identifiers

PMID42548660
PMCPMC13429612

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.