Evidence map›Paper›PMID 42548705›Full record

ArticleFrontiers in veterinary science2026

Porcine epidemic diarrhea virus induces Vero cell mitophagy by regulating ROS-mediated PINK1/Parkin signal pathway.

Yuhe Jin, Ao Sun, Ye Jin, Xinxu Li, Mingyue Han, Haoran Zhang, Hanfan Qiao, Wenli Bai, Yue Zhang, Dahan Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuhe JinCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Ao SunCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Ye JinCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Xinxu LiCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Mingyue HanCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Haoran ZhangCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Hanfan QiaoCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.
Wenli BaiTongliao Animal Disease Prevention and Control Center, Tongliao, China.
Yue ZhangState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou, China.
Dahan YangCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Porcine epidemic diarrhea (PED) is a highly contagious enteric disease caused by the porcine epidemic diarrhea virus (PEDV), with mortality rates exceeding 80% in piglets. While the pathogenesis of PEDV is well-documented, its relationship with mitophagy remains unclear. Methods: We used transmission electron microscopy (TEM) to observe ultrastructural changes in PEDV-infected Vero cells. Western blotting and biochemical assays were employed to analyze mitophagy-related markers (LC3-II, PINK1, Parkin, Beclin1) and oxidative stress indicators (ROS, MDA, antioxidant enzymes). The role of the PINK1/Parkin pathway was further validated through siRNA knockdown and overexpression. Results: PEDV infection was found to trigger PINK1/Parkin-dependent mitophagy, evidenced by the accumulation of damaged mitochondria and upregulation of mitophagy markers. This process was driven by ROS-induced oxidative stress, as ROS scavenging significantly attenuated mitophagy. Furthermore, we demonstrated that mitophagy facilitates PEDV replication, with Parkin expression acting as a critical regulator of this viral-induced autophagic pathway. Discussion: This study reveals that PEDV exploits PINK1/Parkin-mediated mitophagy via ROS accumulation to promote viral replication. These findings provide a scientific basis for understanding PEDV pathogenesis and offer a technical reference for optimizing high-titer virus production in vaccine development.

Indexed as

mitophagyparkinPINK1porcine epidemic diarrhea virusROS

Identifiers

PMID42548705
PMCPMC13429497

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.