ArticleFrontiers in bioengineering and biotechnology2026
Construction and simulation-experimental characterization of a fluorescence image-based finite element model for cell mechanics.
Article in Frontiers in bioengineering and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: A finite element (FE) model was developed to characterize the three-dimensional (3D) heterogeneous structure features of cells, and the influence mechanism of cytoskeleton remodeling on the mechanical properties of cells and the intracellular stress transmission behavior was analyzed. Methods: Human lung adenocarcinoma A549 cells were used in this study, and the cytoskeleton model was constructed by using cytochalasin D (Cyto-D) at concentrations of 0, 0.1, and 1.0 μmol/L. Based on the 3D fluorescence image stacks obtained by confocal laser scanning microscopy (CLSM), combined with the image processing algorithm written in MATLAB, the 3D surface structure of the cells was reconstructed. Atomic force microscopy (AFM) was used to perform nanoindentation experiments to characterize the mechanical parameters of the cells. A multi-body contact FE model of "probe-cytoskeleton-nucleus-culture dish" was constructed based on the ANSYS platform, and the stress distribution and mechanical response of cells under indentation loading were simulated on this basis. Results: With the increase of Cyto-D concentration, the AFM nanoindentation showed that the cell height significantly decreased, while the Young's modulus and adhesion force increased ( Conclusion: The proposed framework provides a feasible approach for integrating fluorescence imaging with FE analysis in cell mechanics research and may serve as a methodological reference for studying the relationship between cytoskeletal organization and cellular mechanical behavior.
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