Evidence map›Paper›PMID 42559531›Full record

ArticleChemical engineering journal (Lausanne, Switzerland : 1996)2026

Bioprinting of miRNA-Induced Spheroids for Vascularized, Heterocellular Bone Regeneration.

Nazmiye Celik, Amar Yeware, Vaibhav Pal, Miji Yeo, Myoung Hwan Kim, Logan Haugh, Ibrahim T Ozbolat, Daniel J Hayes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical engineering journal (Lausanne, Switzerland : 1996), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nazmiye CelikDepartment of Engineering Science and Mechanics, Pennsylvania State University, 212 Earth-Engineering Sciences Bldg., University Park, PA 16802, USA.
Amar YewareDepartment of Biomedical Engineering, Pennsylvania State University, Chemical and Biomedical Engineering Bldg., University Park, PA 16802, USA.
Vaibhav PalThe Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Miji YeoDepartment of Engineering Science and Mechanics, Pennsylvania State University, 212 Earth-Engineering Sciences Bldg., University Park, PA 16802, USA.
Myoung Hwan KimThe Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Logan HaughDepartment of Biomedical Engineering, Pennsylvania State University, Chemical and Biomedical Engineering Bldg., University Park, PA 16802, USA.
Ibrahim T OzbolatDepartment of Engineering Science and Mechanics, Pennsylvania State University, 212 Earth-Engineering Sciences Bldg., University Park, PA 16802, USA.
Daniel J HayesThe Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.

Funding

Intraoperative bioprinting of composite tissues with zonal stratification for craniomaxillofacial reconstructionR01DE028614 · NIDCR · PENNSYLVANIA STATE UNIVERSITY, THE · PI OZBOLAT, IBRAHIM · 2020 to 2024
$2.8M
High-throughput Spheroid Bioprinting Technology for Scalable Fabrication of TissuesR01EB034566 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI Ibrahim Ozbolat · 2023 to 2026
$2.2M
Spatiotemporal Modulation of Osteogenesis in a 3-D Stromal/Stem Cell ModelR01DE024790 · NIDCR · PENNSYLVANIA STATE UNIVERSITY, THE · PI HAYES, DANIEL J. · 2015 to 2019
$1.7M
NIBIB NIH HHS R01 EB034566NIDCR NIH HHS R01 DE024790NIDCR NIH HHS R01 DE028614
6 · The paper itself

Abstract

Successful bone regeneration requires coupled osteogenic and vascular development; however, achieving simultaneous multicellular differentiation within engineered tissues remains challenging. Here, we developed a microRNA (miR)-guided spheroid platform to induce dual osteogenic and endothelial differentiation of human adipose-derived stem cells (hASCs) for vascularized bone regeneration. hASCs were transfected with miR-148b or miR-210 to promote osteogenic and vascular-associated phenotypes, respectively, and assembled into spheroids that were bioprinted within an nHA-containing GelMA microgel environment using aspiration-assisted bioprinting (AAB). The integrated platform combined miR-guided osteogenic and endothelial differentiation, spatially organized AAB-based spheroid assembly, and an nHA-containing GelMA microgel environment to support vascularized bone tissue regeneration. The engineered constructs maintained high cell viability (> 90%) and supported active cell spreading and migration within the microgel matrix, together with increased osteogenic and endothelial gene expression. To further verify their

Indexed as

bioprintingmiRNAosteogenesisspheroidsvascularization

Identifiers

PMID42559531
PMCPMC13441407

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.