ArticleSTAR protocols2026
Protocol for detecting protein recruitment to clathrin structures in cultured cells.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Clathrin-mediated endocytosis internalizes key cell-surface receptors and relies on coordinated recruitment of adaptors and accessory proteins to clathrin structures. Here, we present a protocol for quantifying protein recruitment to diffraction-limited clathrin structures at the plasma membrane. We describe steps for the inducible expression of tagged proteins, total internal reflection fluorescence (TIRF) microscopy, the analysis of proteins within clathrin structures, UltraID-based proximity-labeling, and western blotting detection of proteins in clathrin structures. This protocol is applicable to the detection of any protein within clathrin or related structures. For complete details on the use and execution of this protocol, please refer to Orofiamma et al.
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