Evidence map›Paper›PMID 42561557›Full record

ArticleCellular immunology2026

Validation of a human-specific, multiplex platform for detecting common marmoset (Callithrix jacchus) inflammatory analytes.

Donald R Rogers, Charleston D Berryman, Victor J Rivero, Sydney E Ryan, Shivdeep S Hayer, Mackenzie Conrin, Aliyah Jabenis, Claudia M Rauter, Jonathan D Buhrman, Jonathan B Clayton and 1 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Donald R RogersDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA.
Charleston D BerrymanDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA.
Victor J RiveroDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA.
Sydney E RyanDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.
Shivdeep S HayerDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.
Mackenzie ConrinDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.
Aliyah JabenisDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.
Claudia M RauterDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA.
Jonathan D BuhrmanMeso Scale Diagnostics, LLC, Rockville, MD, USA.
Jonathan B ClaytonDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.; Primate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA.. Electronic address: jclayton@unomaha.edu.
Paul W DentonDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA. Electronic address: pdenton@unomaha.edu.

Funding

UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Heather Colleen Jensen-Smith · 2012 to 2026
$59.2M
Dissecting the Differential Impacts of Toll-like Receptor 9 Agonism on the Capacity of Human Natural Killer Cells to Mediate Target Cell KillingR15AI178516 · NIAID · UNIVERSITY OF NEBRASKA OMAHA · PI PAUL W. DENTON · 2023 to 2026
$743k
A Model of Antibiotic-induced Gut Dysbiosis and Depressive SymptomatologyK01OD030514 · OD · UNIVERSITY OF NEBRASKA OMAHA · PI Jonathan Brent Clayton · 2022 to 2026
$634k
NIAID NIH HHS R15 AI178516NIGMS NIH HHS P20 GM103427NIH HHS K01 OD030514
6 · The paper itself

Abstract

The common marmoset (Callithrix jacchus) is a valuable non-human primate model organism with a long history of use in biomedical research, including investigating diseases with an immune component. However, prior to this effort, there has been a lack of sufficiently validated tools to quantify inflammatory markers in the common marmoset using a multiplex approach. We provide a cross-species reactivity validation of a human-specific multiplex analysis strategy that allows for the assessment of 20 distinct inflammatory analytes in a small volume of plasma isolated from common marmoset blood. We used this novel approach to perform an assessment of the impact of sex as a biological variable on a subset of these inflammatory analytes. We found that endogenous plasma levels of the following analytes are not significantly different between female and male marmosets: CRP, ICAM-1, VCAM-1, IL-13, IL-8, MIP-1β, IFN-g, IL-4, VEGF-A, FLT-1, and bFGF. These findings demonstrate the utility of our validated method for quantifying marmoset inflammatory markers.

Indexed as

CallithrixInflammationAnimalsBiomarkersC-Reactive ProteinCytokinesFemaleFibroblast Growth Factor 2HumansIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-8MaleSpecies SpecificityVascular Cell Adhesion Molecule-1Vascular Endothelial Growth Factor ABiomarkersC-Reactive ProteinCytokinesFibroblast Growth Factor 2Intercellular Adhesion Molecule-1Interferon-gammaInterleukin-8Vascular Cell Adhesion Molecule-1Vascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Cross-reactiveInflammationMarmosetMultiplexPlasmaSex differencesValidation

Identifiers

PMID42561557
PMCPMC13561577

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.