Evidence map›Paper›PMID 42564856›Full record

ArticleRSC chemical biology2026

Illuminating spatial dynamics of glutamine metabolism with a sensitive genetically encoded biosensor.

Jack M Scully, Michael J Sun, Siddharth Das, Nataly J Arias, Edmund D Kapelczak, Tiffany D Robinson, Katie G Vineall, Teagan S Dean, Tara TeSlaa, Ajit S Divakaruni and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Jack M ScullyDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.ORCID https://orcid.org/0009-0006-1524-7884
Michael J SunDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.
Siddharth DasDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.
Nataly J AriasDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles Los Angeles California 90095 USA.
Edmund D KapelczakDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles Los Angeles California 90095 USA.
Tiffany D RobinsonDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.
Katie G VineallDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.
Teagan S DeanDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.
Tara TeSlaaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles Los Angeles California 90095 USA.
Ajit S DivakaruniDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles Los Angeles California 90095 USA.
Danielle L SchmittDepartment of Chemistry and Biochemistry, University of California Los Angeles Los Angeles California 90095 USA.ORCID https://orcid.org/0000-0002-7343-2125

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DENNIS J SLAMON · 1985 to 2026
$134.5M
Research Training in Cell and Molecular BiologyT32GM145388 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Jorge Torres · 2022 to 2026
$5.2M
Understanding How Metabolic Cofactors Control Cell Function and FateR35GM138003 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Ajit Divakaruni · 2020 to 2026
$2.8M
Imaging Spatiotemporal Regulation of Acetyl-CoADP2GM154012 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Danielle Lynanne Schmitt · 2023 to 2026
$2.2M
Stopped-Flow SpectrometerS10OD023584 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YEATES, TODD O · 2017 to 2017
$106k
NCI NIH HHS P30 CA016042NIGMS NIH HHS DP2 GM154012NIGMS NIH HHS R35 GM138003NIGMS NIH HHS T32 GM145388NIH HHS S10 OD023584
6 · The paper itself

Abstract

Glutamine is the most abundant amino acid in serum, used as a key nutrient by cells for protein synthesis, energy production, carbon and nitrogen metabolism, and cellular redox balance. The use of glutamine in the cell is highly compartmentalized, but the dynamics of glutamine metabolism across organelles and individual cells are not fully understood. To illuminate subcellular glutamine dynamics, we developed a green fluorescent protein-based intracellular glutamine optical reporter, iGlo. We find iGlo is sensitive and specific for glutamine and can be used to measure glutamine uptake, production, and consumption with high spatiotemporal resolution in multiple cell types. Furthermore, multiplexed imaging of iGlo with a lactate biosensor in single cells reveals the temporal crosstalk between glucose and glutamine metabolism to maintain energy homeostasis. Thus, iGlo enables the sensitive and precise study of compartmentalized glutamine dynamics and represents a new and enhanced tool for studying the spatiotemporal dynamics and regulation of metabolism.

Identifiers

PMID42564856
PMCPMC13446454

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.