Evidence map›Paper›PMID 42565280›Full record

ArticleJournal of cell science2026

Identification of feeding apparatus components in a heterotrophic marine flagellate.

Gillian Clifford, Samuel J P Taylor, Midori Ishii, Fernanda Cisneros-Soberanis, Bungo Akiyoshi

Abstract read
In one paragraph

Article in Journal of cell science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Gillian CliffordCentre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0009-0001-5258-4293
Samuel J P TaylorCentre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0002-0654-619X
Midori IshiiCentre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0001-9597-9458
Fernanda Cisneros-SoberanisCentre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0003-1120-1695
Bungo AkiyoshiCentre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0001-6010-394X

Funding

University of EdinburghWellcome TrustWellcome Trust 226791Wellcome Trust 227243/Z/23/Z
6 · The paper itself

Abstract

Diplonemids are highly abundant heterotrophic single-celled flagellates that are widespread throughout the oceans of the world. They have a highly complex microtubule-based feeding apparatus (the cytostome-cytopharynx complex) located adjacent to the deep flagellar pocket from which two flagella emerge. The apical papilla is a tongue-shaped structure unique to diplonemids that connects the cytopharynx and the flagellar pocket, the latter of which is formed by reinforcing microtubules (MTR) and two flagellar roots called intermediate and dorsal roots. Here, we report identification of 17 proteins that localize at the feeding apparatus or flagellar apparatus in Diplonema papillatum. Using ultrastructure expansion microscopy, we show that Mad2 and its interaction partner MBP65 localize at the MTR, intermediate root and dorsal root. Homologs of proteins that associate with the flagellar apparatus in Trypanosoma brucei (PFR2, KMP-11 and BILBO1) localize at the feeding apparatus in D. papillatum. We also identify proteins that localize at the apical papilla, MTR, parallel microtubule loop and cytopharynx. By discovering many components of the feeding apparatus in diplonemids, this work forms the foundation to understand the feeding mechanism in these highly abundant marine planktonic organisms.

Indexed as

FlagellaHeterotrophic ProcessesProtozoan ProteinsMicrotubulesProtozoan ProteinsCytopharynxCytostomeDiplonemidsFeeding apparatusFlagellar rootsParadiplonema papillatum

Identifiers

PMID42565280
PMCPMC13615690

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.