ArticleFrontiers in nutrition2026
Comparative profiling of amino acids in plasma and serum across children and adults using LC-MS/MS and its implications for precision nutrition.
Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: Circulating amino acids (AAs) are important biomarkers of nutritional status, metabolic homeostasis, and disease related physiological changes. However, the influence of specimen type and age on AA measurements remains incompletely understood. This study aimed to establish a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 29 amino acids (AAs), compare serum and plasma matrices, and investigate age related variations in healthy populations. Methods: In a cross-sectional study, a total of 231 healthy adults (≥18 years) and 140 children (<18 years) were recruited from the Shanghai area with a subgroup of 26 adults providing paired serum and plasma samples. Twenty-nine AA quantification was performed using LC-MS/MS with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization. Method validation followed Clinical and Laboratory Standards Institute (CLSI) guidelines for linearity, lower limit of quantitation (LLOQ), precision, accuracy, carryover, and stability. Results: The assay demonstrated excellent linearity ( Conclusion: The validated LC-MS/MS platform provides a reliable platform for nutritional biomarker research. Matrix selection and age are critical factors in AA interpretation, offering valuable insights for clinical diagnostics and translational metabolomics research.
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