Evidence map›Paper›PMID 42573698›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

An Auxin-Inducible Degron System for Trypanosomes.

Alexandra Klein, Alice McDowell, Christian R S Reis, Rodrigo Pontes de Lima, Danielle M N Moura, Janaina de Freitas Nascimento, Osvaldo P de Melo Neto, Mark Carrington

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alexandra KleinDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Alice McDowellDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Christian R S ReisDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Rodrigo Pontes de LimaDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Danielle M N MouraDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Janaina de Freitas NascimentoDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Osvaldo P de Melo NetoAggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil.
Mark CarringtonDepartment of Biochemistry, University of Cambridge, Cambridge, UK. mc115@cam.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability to specifically degrade one protein in a cell provides an immediate insight into any resulting phenotype and thus function that may be occluded by secondary effects using other techniques. The auxin-inducible degron is a method developed to achieve this end and is effective in mammalian and yeast cells. The approach is dependent on the recognition of a tagged target protein by an endogenous E3 ubiquitin ligase modified by the expression of a plant F-box protein. This approach was initially unsuccessful in trypanosomes, probably due to a lack of interaction between the endogenous E3 ubiquitin ligase and the plant F-box protein. This was overcome by expressing the entire rice E3 ubiquitin ligase in trypanosomes, and here we describe the production of the auxin-inducible degron competent cell lines and tagging of target genes.

Indexed as

Indoleacetic AcidsTrypanosomaTrypanosoma brucei bruceiDegronsF-Box ProteinsOryzaPlant ProteinsProteolysisUbiquitin-Protein LigasesF-Box ProteinsIndoleacetic AcidsPlant ProteinsUbiquitin-Protein LigasesAuxin-inducible degronTrypanosome

Identifiers

PMID42573698

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.