Evidence map›Paper›PMID 42578665›Full record

ArticleJournal of virology2026

A nanoluciferase-expressing attenuated CHIKV vaccine strain enables rapid and visual drug screening

Mengyuan Zheng, Zhouling Pan, Yanxian Jiang, Yan Guo, Wei Chang, Hailin Tang, Xiao Chen, Yujie Xiang, Xue Wang, Zihao Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Mengyuan Zheng *Faculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Zhouling Pan *Faculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Yanxian JiangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Yan GuoFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Wei ChangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Hailin TangDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Xiao ChenFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Yujie XiangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Xue WangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Zihao WangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Ranran ChangFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Li LiuFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.
Ping ZhaoDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.ORCID 0000-0002-1289-326X
Yue FengFaculty of Life Science and Technology & Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID 0009-0007-4497-6944
Xueshan XiaYunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming Medical University, Kunming, Yunnan, China.ORCID 0000-0003-1120-8870

Funding

Yunnan Major Scientific and Technological Projects 202202AG050013, 202302AA310044, 202405AJ310002, 202502AA310014
6 · The paper itself

Abstract

Climate change has expanded the range of vectors for the chikungunya virus (CHIKV), thereby increasing its global threat. This situation underscores the urgent need for antiviral therapies. However, the development of drugs is limited by the need for Biosafety Level 3 (BSL-3) containment and the lack of tools for real-time infection monitoring. To address these challenges, we developed a replication-competent reporter virus called CHIKV-ΔnsP3-Nluc. We inserted the Nano-luciferase (Nluc) gene into the attenuation site of the FDA-approved live-attenuated vaccine strain VLA1553 (IXCHIQ). This modified virus retains an attenuated phenotype, replicating more slowly than wild-type CHIKV while maintaining high genetic stability. Nluc activity correlates well with viral titer (

Indexed as

Antiviral AgentsChikungunya FeverChikungunya virusLuciferasesViral VaccinesAnimalsChlorocebus aethiopsDrug Evaluation, PreclinicalFemaleGenes, ReporterHumansMiceVaccines, AttenuatedVirus ReplicationAntiviral AgentsLuciferasesVaccines, AttenuatedViral Vaccines4'-fluorouridinechikungunya virusdrug screeningnano-luciferasereporter virusVLA1553

Identifiers

PMID42578665
PMCPMC13595972

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.